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Antibodies > TUSC3 > LS-B13274
 
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Catalog Number Size Price
LS-B13274-50 50 µl (1 mg/ml) $460 
TUSC3 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
TUSC3 Antibody - Immunohistochemical analysis of TUSC3 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
TUSC3 Antibody - Immunofluorescent analysis of TUSC3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
TUSC3 Antibody - Western blot analysis of TUSC3 expression in SKBR3 (A); HeLa (B); HepG2 (C); MDAMB231 (D) whole cell lysates.
TUSC3 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
TUSC3 Antibody - Immunohistochemical analysis of TUSC3 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
TUSC3 Antibody - Immunofluorescent analysis of TUSC3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
TUSC3 Antibody - Western blot analysis of TUSC3 expression in SKBR3 (A); HeLa (B); HepG2 (C); MDAMB231 (D) whole cell lysates.
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IHC‑plus™ Polyclonal Rabbit anti‑Human TUSC3 Antibody (Internal, IHC, IF, WB) LS‑B13274

IHC‑plus™ Polyclonal Rabbit anti‑Human TUSC3 Antibody (Internal, IHC, IF, WB) LS‑B13274

IHC Validated
(5.0) Rating
Submit Review (1) Publication(s) LSBio Guarantee
Note: This antibody replaces LS-C353027
Antibody:
TUSC3 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Bovine
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B13274-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
TUSC3 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Bovine
Format:
Unconjugated, Unmodified

Specifications

Description
TUSC3 antibody LS-B13274 is an unconjugated rabbit polyclonal antibody to TUSC3 (Internal) from human. It is reactive with human, bovine and monkey. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
(1) Publication(s)
Target
Human TUSC3
Synonyms
TUSC3 | D8S1992 | M33 | MRT7 | N33 | OST3A | Tumor suppressor candidate 3 | Protein N33 | MRT22
Host
Rabbit
Reactivity
Human, Monkey, Bovine (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human TUSC3.
Epitope
Internal
Specificity
Recognizes endogenous levels of TUSC3 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TUSC3
Q13454 NM_006765 NP_006756.2

Validation

TUSC3 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
About LSBio Validation process

LSBio Ratings

IHC-plus™ TUSC3 Antibody (Internal) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-B13274 has an LSBio Rating of
(5.0) Rating
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Low levels of tumor suppressor candidate 3 predict poor prognosis of patients with hepatocellular carcinoma. Sheng XR, Xing SG, Wang RD, Chen K, Jia WD. OncoTargets and therapy. 2018 February;11:909-917. (Human)
Full Text Article PubMed: 29503566 PMC: PMC5825997
Submit a Publication for LS-B13274

Customer Reviews (0)

Submit a review for LS-B13274

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
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