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Anti-TUBG1 / Tubulin Gamma 1 Antibody (C-Terminus) IHC-plus™ LS-B1496

Note: This antibody replaces LS-C41288

Ordering

Wt. Vol. Conc. Price
- 200 µl 0.2 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular TUBG1 / Tubulin Gamma 1 Antibodies

Anti-TUBG1 / Tubulin Gamma 1 Antibody (C-Terminus) IHC-plus™ LS-B1496
Rabbit Polyclonal (IgG) to Human TUBG1 / Tubulin Gamma 1
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-TUBG1 / Tubulin Gamma 1 Antibody (aa432-451) LS-C50251
Rabbit Polyclonal to Human TUBG1 / Tubulin Gamma 1
Human, Mouse, Rat
IHC, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (2)
Rabbit Polyclonal (IgG) to Human TUBG1 / Tubulin Gamma 1
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human TUBG1 / Tubulin Gamma 1
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • Immunofluorescence
  • Western blot

Specificity and Use

TUBG1 / Tubulin Gamma 1 antibody was raised against synthetic peptide from human TUBG1 / Gamma Tubulin.
C-Terminus
Peptide-KLH
Immunohistochemistry: LS-B1496 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1496 was determined to be 1:50.

Packaging

Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 50% glycerol.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (2)

1
Structure-specific recognition protein 1 facilitates microtubule growth and bundling required for mitosis. Zeng SX, Li Y, Jin Y, Zhang Q, Keller DM, McQuaw CM, Barklis E, Stone S, Hoatlin M, Zhao Y, Lu H. Molecular and cellular biology. 2010 30:935-47. [PubMed:19995907] [PMC:PMC2815565]
2
Aurora kinase A drives MTOC biogenesis but does not trigger resumption of meiosis in mouse oocytes matured in vivo. Solc P, Baran V, Mayer A, Bohmova T, Panenkova-Havlova G, Saskova A, Schultz RM, Motlik J. Biology of reproduction. 2012 87:85. [PubMed:22837479] [PMC:PMC3507544]

About TUBG1 / Tubulin Gamma 1

P23258 NM_001070 NP_001061.2

TUBG1 Antibody, Gamma-1-tubulin Antibody, GCP-1 Antibody, Gamma Tubulin Antibody, TUBGCP1 Antibody, Tubulin gamma chain Antibody, TUBG Antibody, Tubulin, gamma polypeptide Antibody, Tubulin gamma-1 chain Antibody, Tubulin, gamma 1 Antibody

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.

Immunohistochemistry

Immunohistochemistry
Anti-TUBG1 / Gamma Tubulin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1496 dilution 1:50.

Immunocytochemistry

Immunocytochemistry
Overnight nocodazole treated Hela cells stained with purified rabbit polyclonal antibody against -tubulin, followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI.

Western blot

Western blot
Whole cell extract from A431 cells (Lane 1) and NIH3T3 cells (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti--tubulin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
 

Requested From: United States
Date Requested: 12/5/2016

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