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Antibodies > TUBA1B / Tubulin Alpha 1B > LS-B10591
 
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Catalog Number Size Price
LS-B10591-50 50 µg (1 mg/ml) $485 
TUBA1B / Tubulin Alpha 1B Antibody - Anti-TUBA1B antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
TUBA1B / Tubulin Alpha 1B Antibody - Anti-alpha-Tubulin Monoclonal Antibody - Immunohistochemistry. anti-a-tubulin monoclonal antibody was used at a 2.5 ug/mL to detect tubulin in thyroid follicular epithelium (40X) showing moderate to strong cytoplasmic staining (image). Moderate to strong cytoplasmic staining was also observed within subsets of neurons and glia, and epithelial cells including adrenal, breast, colon, pancreas, kidney, prostate, placenta, skin, testis, uterus, thyroid, and within lymphoid organs. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. tissue was formalin-fixed and paraffin embedded.
TUBA1B / Tubulin Alpha 1B Antibody - Anti-tubulin Monoclonal antibody-Immunofluorescence. mouse monoclonal anti-tubulin antibody was used with ATTO 425 Goat anti-mouse ( shown in red) to detect tubulin by immunofluorescence. Dylight 488 Goat anti-Rabbit (shown in green) was used in the same experiment to detect rabbit anti-Clathrin polyclonal antibody Data was collected on a STED-CW TCS-SP5 Confocal system (Leica Microsystems) equipped with a DFC 350FX Camera allowing sequential acquisition in wide-field, confocal and STED CW imaging modes and provided courtesy of: Myriam Gastard, PhD, personal communication, Leica Microsystems, Inc. USA.
TUBA1B / Tubulin Alpha 1B Antibody - Immunofluorescence Microscopy - alpha-Tubulin Monoclonal Antibody -. a-tubulin monoclonal antibody detects tubulin (colored RED) in STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 ug/mL final concentration of anti-a-tubulin and detected with 0.2 ug/mL ATTO 425 conjugated anti-MOUSE IgG [GOAT] ( secondary antibody. Also shown in this 2-color STED image is Anti-HDAC-1 [RABBIT] (LS-B34) detected with DyLight488 conjugated Anti-RABBIT IgG [GOAT] secondary antibody (colored GREEN). Image courtesy of Myriam Gastard, Leica Microsystems, USA.
TUBA1B / Tubulin Alpha 1B Antibody - Anti-alpha-Tubulin Monoclonal Antibody - Immunofluorescence Microscopy. anti-a-tubulin monoclonal antibody was used at a 0.1 ug/mL to detect tubulin in 4% paraformaldehyde fixed A459 cells. Staining is shown using conventional confocal microscopy (left panel) and by high resolution TCS STED nanoscopy (right panel). DyLight488 conjugated anti-mouse IgG secondary antibody (p/n was used for detection at 1 ug/mL. Personal Communication, Myriam. Gastard, Leica Microsystems, Exton PA.
TUBA1B / Tubulin Alpha 1B Antibody - Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody - Immunofluorescence. Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody Immunofluorescence image showing MCF-7 cell staining of Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody in green and staining of Anti-Gli-3 (RABBIT) Antibody in red.
TUBA1B / Tubulin Alpha 1B Antibody - Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody - Immunofluorescence. Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody Immunofluorescence image one showing MCF-7 cell staining of Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody in green. Immunofluorescence image two showing MCF-7 cell staining of Anti-Gli-3 (RABBIT) Antibody in red. Immunofluorescence image three showing MCF-7 cell superimposed staining of Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody in green and staining of Anti-Gli-3 (RABBIT) antibody in red.
TUBA1B / Tubulin Alpha 1B Antibody - Anti-alpha-Tubulin Monoclonal Antibody - Western Blot. HeLa whole cell lysate (left lane) and HEK293 whole cell lysate (right lane) were loaded with 10 ug of lysate each. The blot was blocked with Blocking Buffer (MB-070) for 30 min at RT, then washed and incubated with anti-Tubulin monoclonal antibody diluted in Blocking Buffer (p/n MB-070) at 1:1000 for 1 h at RT. After washing, blot was incubated with a 1:40000 dilution of HRP Rb a-Ms IgG (p/n LS-C60772) secondary antibody in Blocking Buffer (p/n MB-070) for 30 minutes at RT. Data was collected using Bio-Rad VersaDoc 4000 MP.
TUBA1B / Tubulin Alpha 1B Antibody - Anti-TUBA1B antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
TUBA1B / Tubulin Alpha 1B Antibody - Anti-alpha-Tubulin Monoclonal Antibody - Immunohistochemistry. anti-a-tubulin monoclonal antibody was used at a 2.5 ug/mL to detect tubulin in thyroid follicular epithelium (40X) showing moderate to strong cytoplasmic staining (image). Moderate to strong cytoplasmic staining was also observed within subsets of neurons and glia, and epithelial cells including adrenal, breast, colon, pancreas, kidney, prostate, placenta, skin, testis, uterus, thyroid, and within lymphoid organs. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. tissue was formalin-fixed and paraffin embedded.
TUBA1B / Tubulin Alpha 1B Antibody - Anti-tubulin Monoclonal antibody-Immunofluorescence. mouse monoclonal anti-tubulin antibody was used with ATTO 425 Goat anti-mouse ( shown in red) to detect tubulin by immunofluorescence. Dylight 488 Goat anti-Rabbit (shown in green) was used in the same experiment to detect rabbit anti-Clathrin polyclonal antibody Data was collected on a STED-CW TCS-SP5 Confocal system (Leica Microsystems) equipped with a DFC 350FX Camera allowing sequential acquisition in wide-field, confocal and STED CW imaging modes and provided courtesy of: Myriam Gastard, PhD, personal communication, Leica Microsystems, Inc. USA.
TUBA1B / Tubulin Alpha 1B Antibody - Immunofluorescence Microscopy - alpha-Tubulin Monoclonal Antibody -. a-tubulin monoclonal antibody detects tubulin (colored RED) in STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 ug/mL final concentration of anti-a-tubulin and detected with 0.2 ug/mL ATTO 425 conjugated anti-MOUSE IgG [GOAT] ( secondary antibody. Also shown in this 2-color STED image is Anti-HDAC-1 [RABBIT] (LS-B34) detected with DyLight488 conjugated Anti-RABBIT IgG [GOAT] secondary antibody (colored GREEN). Image courtesy of Myriam Gastard, Leica Microsystems, USA.
TUBA1B / Tubulin Alpha 1B Antibody - Anti-alpha-Tubulin Monoclonal Antibody - Immunofluorescence Microscopy. anti-a-tubulin monoclonal antibody was used at a 0.1 ug/mL to detect tubulin in 4% paraformaldehyde fixed A459 cells. Staining is shown using conventional confocal microscopy (left panel) and by high resolution TCS STED nanoscopy (right panel). DyLight488 conjugated anti-mouse IgG secondary antibody (p/n was used for detection at 1 ug/mL. Personal Communication, Myriam. Gastard, Leica Microsystems, Exton PA.
TUBA1B / Tubulin Alpha 1B Antibody - Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody - Immunofluorescence. Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody Immunofluorescence image showing MCF-7 cell staining of Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody in green and staining of Anti-Gli-3 (RABBIT) Antibody in red.
TUBA1B / Tubulin Alpha 1B Antibody - Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody - Immunofluorescence. Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody Immunofluorescence image one showing MCF-7 cell staining of Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody in green. Immunofluorescence image two showing MCF-7 cell staining of Anti-Gli-3 (RABBIT) Antibody in red. Immunofluorescence image three showing MCF-7 cell superimposed staining of Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody in green and staining of Anti-Gli-3 (RABBIT) antibody in red.
TUBA1B / Tubulin Alpha 1B Antibody - Anti-alpha-Tubulin Monoclonal Antibody - Western Blot. HeLa whole cell lysate (left lane) and HEK293 whole cell lysate (right lane) were loaded with 10 ug of lysate each. The blot was blocked with Blocking Buffer (MB-070) for 30 min at RT, then washed and incubated with anti-Tubulin monoclonal antibody diluted in Blocking Buffer (p/n MB-070) at 1:1000 for 1 h at RT. After washing, blot was incubated with a 1:40000 dilution of HRP Rb a-Ms IgG (p/n LS-C60772) secondary antibody in Blocking Buffer (p/n MB-070) for 30 minutes at RT. Data was collected using Bio-Rad VersaDoc 4000 MP.
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IHC‑plus™ Monoclonal Mouse anti‑Human TUBA1B / Tubulin Alpha 1B Antibody (clone 17H11.F10, C‑Terminus, IHC, IF, WB) LS‑B10591

IHC‑plus™ Monoclonal Mouse anti‑Human TUBA1B / Tubulin Alpha 1B Antibody (clone 17H11.F10, C‑Terminus, IHC, IF, WB) LS‑B10591

IHC Validated
(4.0) Rating
Submit Review LSBio Guarantee
Note: This antibody replaces LS-C154003
Antibody:
TUBA1B / Tubulin Alpha 1B Mouse anti-Human Monoclonal (C-Terminus) (17H11.F10) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B10591-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
TUBA1B / Tubulin Alpha 1B Mouse anti-Human Monoclonal (C-Terminus) (17H11.F10) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
Tubulin Alpha 1B antibody LS-B10591 is an unconjugated mouse monoclonal antibody to Tubulin Alpha 1B (TUBA1B) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TUBA1B / Tubulin Alpha 1B
Synonyms
TUBA1B | Alpha-tubulin ubiquitous | Alpha tubulin | K-ALPHA-1 | Tubulin, alpha 1b | Tubulin alpha-ubiquitous chain | Tubulin, alpha, ubiquitous | Tubulin K-alpha-1 | Tubulin alpha chain | Tubulin alpha-1B chain
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine, Chicken (tested or 100% immunogen sequence identity)
Predicted
Fish, Algae, Avian, Bacteria, Mammal, Protozoa (at least 90% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
17H11.F10
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.
Epitope
C-Terminus
Specificity
A BLAST analysis was used to suggest antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (0.1 µg/ml)
  • Western blot (1:1000)
  • ELISA (1:300000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kD in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TUBA1B / Tubulin Alpha 1B
P68363 NM_006082 NP_006073.2

Validation

TUBA1B / Tubulin Alpha 1B Antibody - Anti-TUBA1B antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TUBA1B antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TUBA1B antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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About LSBio Validation process

LSBio Ratings

IHC-plus™ TUBA1B / Tubulin Alpha 1B Antibody (C-Terminus, clone 17H11.F10) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B10591 has an LSBio Rating of
(4.0) Rating
Laboratory Validation Score (4)

Publications (0)

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Customer Reviews (0)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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