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Anti-TSPAN12 Antibody (aa232-259) LS-C160262

Ordering

Wt. Vol. Conc. Price
- 400 µl - Unavailable

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Anti-TSPAN12 Antibody (aa232-259) LS-C160262
Rabbit Polyclonal to Human TSPAN12
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-TSPAN12 Antibody (aa185-234) IHC-plus™ LS-B9720
Rabbit Polyclonal (IgG) to Human TSPAN12
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-TSPAN12 Antibody (aa185-234, Biotin) LS-C449404
Rabbit Polyclonal (IgG) to Human TSPAN12
Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
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Rabbit Polyclonal to Human TSPAN12
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human TSPAN12
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Rat, Cow (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

LS-E9728 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C160262. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa232-259
This TSPAN12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232-259 amino acids from the C-terminal region of human TSPAN12.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About TSPAN12

O95859 NM_012338 NP_036470.1

TSPAN12 Antibody, EVR5 Antibody, NET-2 Antibody, NET2 Antibody, Tetraspan NET-2 Antibody, Tetraspanin-12 Antibody, TM4SF12 Antibody, Tspan-12 Antibody, Tetraspanin 12 Antibody

Regulator of cell surface receptor signal transduction. Plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. Acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulate LEF/TCF-mediated transcriptional programs.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human brain tissue reacted with TSPAN12 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot
Western blot of TSPAN12 Antibody in T47D cell line lysates (35 ug/lane). TSPAN12 (arrow) was detected using the purified antibody.

Flow Cytometry

Flow Cytometry
TSPAN12 Antibody flow cytometry of 293 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
 

Requested From: 
Date Requested: 3/26/2017

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