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Anti-TSPAN12 Antibody (aa232-259) LS-C160262

Catalog Size Price
LS-C160262-400 400 µl Unavailable

Related Products

21 TSPAN12 Antibodies

Most Popular TSPAN12 Antibodies

Anti-TSPAN12 Antibody (aa232-259) LS-C160262
Rabbit Polyclonal to Human TSPAN12
Human
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Formalin-fixed and paraffin-embedded human brain tissue reacted with TSPAN12 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Anti-TSPAN12 Antibody (aa185-234) IHC-plus™ LS-B9720
Rabbit Polyclonal (IgG) to Human TSPAN12
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-TSPAN12 antibody IHC staining of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9720 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-TSPAN12 Antibody (aa232-259) LS-C328870
Rabbit Polyclonal (IgG) to Human TSPAN12
Human
Western blot, ELISA
Unconjugated
Anti-TSPAN12 Antibody LS-C662792
Rabbit Polyclonal to Human TSPAN12
Human
IHC, Western blot, ELISA
Unconjugated
Immunohistochemistry - Anti-TSPAN12 Picoband antibody
Anti-TSPAN12 Antibody LS-C681623
Rabbit Polyclonal (IgG) to Human TSPAN12
Human
IHC, Immunofluorescence, ELISA
Unconjugated
Immunohistochemistry Dilution at 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

100% Guaranteed
Rabbit Polyclonal to Human TSPAN12
Human
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human TSPAN12
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Rat, Cow (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

LS-E9728 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C160262. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa232-259
This TSPAN12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232-259 amino acids from the C-terminal region of human TSPAN12.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About TSPAN12

O95859 NM_012338 NP_036470.1

TSPAN12 Antibody, EVR5 Antibody, NET-2 Antibody, NET2 Antibody, Tetraspan NET-2 Antibody, Tetraspanin-12 Antibody, TM4SF12 Antibody, Tspan-12 Antibody, Tetraspanin 12 Antibody

Regulator of cell surface receptor signal transduction. Plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. Acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulate LEF/TCF-mediated transcriptional programs.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with TSPAN12 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with TSPAN12 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of TSPAN12 Antibody in T47D cell line lysates (35 ug/lane). TSPAN12 (arrow) was detected using the purified antibody.
Western blot of TSPAN12 Antibody in T47D cell line lysates (35 ug/lane). TSPAN12 (arrow) was detected using the purified antibody.

Flow Cytometry

TSPAN12 Antibody flow cytometry of 293 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TSPAN12 Antibody flow cytometry of 293 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: 
Date Requested: 4/26/2018

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