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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TSPAN12 Antibody (aa179‑228) LS‑C111131
TSPAN12 antibody LS-C111131 is an unconjugated rabbit polyclonal antibody to TSPAN12 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C111131-50
50 µg
Unavailable

Popular TSPAN12 Products

Formalin-fixed and paraffin-embedded human brain tissue reacted with TSPAN12 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Anti-TSPAN12 antibody IHC staining of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9720 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Anti-TSPAN12 antibody IHC staining of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9720 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry - Anti-TSPAN12 Picoband antibody
Species: Human
Applications: IHC, Western blot, ELISA
Immunohistochemistry Dilution at 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, Immunofluorescence, ELISA

Product Description

TSPAN12 antibody LS-C111131 is an unconjugated rabbit polyclonal antibody to TSPAN12 from human, mouse, rat and other species. Validated for WB.
About TSPAN12
Regulator of cell surface receptor signal transduction. Plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. Acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulate LEF/TCF-mediated transcriptional programs. O95859 NM_012338 NP_036470.1

TSPAN12 Antibody, EVR5 Antibody, NET-2 Antibody, NET2 Antibody, Tetraspan NET-2 Antibody, Tetraspanin-12 Antibody, TM4SF12 Antibody, Tspan-12 Antibody, Tetraspanin 12 Antibody


Specifications

Target
Human TSPAN12
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
TSPAN12 antibody was raised against synthetic peptide located between aa179-228 of human TSPAN12 (O95859, NP_036470). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%); Turkey, Zebra finch, Chicken, Platypus, Lizard (92%); Zebrafish (84%).
Blocking Peptide
LS-E17055 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C111131. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa179-228
Specificity
Human TSPAN12
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

TSPAN12 antibody LS-C111131 Western blot of Fetal Thymus lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TSPAN12 antibody LS-C111131 Western blot of Fetal Thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TSPAN12 antibody LS-C111131 Western blot of Fetal Thymus lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TSPAN12 antibody LS-C111131 Western blot of Fetal Thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TSPAN12 antibody LS-C111131 Western blot of Fetal Thymus lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TSPAN12 antibody LS-C111131 Western blot of Fetal Thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TSPAN12 antibody LS-C111131 Western blot of Fetal Thymus lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TSPAN12 antibody LS-C111131 Western blot of Fetal Thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/25/2018

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