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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TSHB / TSH‑Beta Antibody (aa58‑86) LS‑C160252
TSH-Beta antibody LS-C160252 is an unconjugated rabbit polyclonal antibody to human TSH-Beta (TSHB). Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C160252-400
400 µl
Unavailable

Popular TSHB / TSH-Beta Products

Antibody
Species: Mouse
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot
Antibody
Species: Rat
Applications: IHC, IHC - Resin, Western blot
Antibody
Species: Human, Fish
Applications: IHC, IHC - Frozen, Western blot
Antibody
Species: Human
Applications: IHC, IHC - Frozen, Western blot
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

TSH-Beta antibody LS-C160252 is an unconjugated rabbit polyclonal antibody to human TSH-Beta (TSHB). Validated for Flow, IF, IHC and WB.
About TSHB / TSH-Beta
The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. Thyroid stimulating hormone functions in the control of thyroid structure and metabolism. P01222 NM_000549 NP_000540.2

TSHB Antibody, Thyrotropin alfa Antibody, Thyrotropin beta subunit Antibody, TSH Beta Antibody, TSH-B Antibody, Thyrotropin beta chain Antibody, Thyrotropin subunit beta Antibody, TSH-BETA Antibody, CHNG4 Antibody


Specifications

Target
Human TSHB / TSH-Beta
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E9678 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C160252. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa58-86
Specificity
This TSHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the Central region of human TSHB.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TSHB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TSHB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Confocal immunofluorescence of TSHB Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of TSHB Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of TSHB Antibody in MDA-MB231 cell line lysates (35 ug/lane). TSHB (arrow) was detected using the purified antibody.
Western blot of TSHB Antibody in MDA-MB231 cell line lysates (35 ug/lane). TSHB (arrow) was detected using the purified antibody.

Flow Cytometry

TSHB Antibody flow cytometry of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TSHB Antibody flow cytometry of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TSHB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TSHB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Confocal immunofluorescence of TSHB Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of TSHB Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of TSHB Antibody in MDA-MB231 cell line lysates (35 ug/lane). TSHB (arrow) was detected using the purified antibody.
Western blot of TSHB Antibody in MDA-MB231 cell line lysates (35 ug/lane). TSHB (arrow) was detected using the purified antibody.

Flow Cytometry

TSHB Antibody flow cytometry of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TSHB Antibody flow cytometry of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TSHB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TSHB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Confocal immunofluorescence of TSHB Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of TSHB Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of TSHB Antibody in MDA-MB231 cell line lysates (35 ug/lane). TSHB (arrow) was detected using the purified antibody.
Western blot of TSHB Antibody in MDA-MB231 cell line lysates (35 ug/lane). TSHB (arrow) was detected using the purified antibody.

Flow Cytometry

TSHB Antibody flow cytometry of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TSHB Antibody flow cytometry of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TSHB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TSHB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Confocal immunofluorescence of TSHB Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of TSHB Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of TSHB Antibody in MDA-MB231 cell line lysates (35 ug/lane). TSHB (arrow) was detected using the purified antibody.
Western blot of TSHB Antibody in MDA-MB231 cell line lysates (35 ug/lane). TSHB (arrow) was detected using the purified antibody.

Flow Cytometry

TSHB Antibody flow cytometry of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TSHB Antibody flow cytometry of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/19/2018

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