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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TSAP6 / STEAP3 Antibody (aa411‑460) LS‑C80575
STEAP3 antibody LS-C80575 is an unconjugated rabbit polyclonal antibody to STEAP3 (TSAP6) from human, bat, guinea pig and other species. Validated for WB.
Catalog
Size
Price
LS-C80575-50
50 µg (1 mg/ml)
Unavailable

Popular TSAP6 / STEAP3 Products

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Immunohistochemical analysis of STEAP3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Product Description

STEAP3 antibody LS-C80575 is an unconjugated rabbit polyclonal antibody to STEAP3 (TSAP6) from human, bat, guinea pig and other species. Validated for WB.
About TSAP6 / STEAP3
Endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe3+ to Fe2+. Can also reduce of Cu2+ to Cu1+, suggesting that it participates in copper homeostasis. Uses NADP+ as acceptor. May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP. Q658P3 NM_018234 NP_060704.2

STEAP3 Antibody, Dudulin-2 Antibody, Dudlin 2 Antibody, Dudlin-2 Antibody, Dudulin 2 Antibody, Metalloreductase STEAP3 Antibody, HTSAP6 Antibody, PHyde Antibody, Tumor suppressor pHyde Antibody, TSAP6 Antibody, HpHyde Antibody, STMP3 Antibody


Specifications

Target
Human TSAP6 / STEAP3
Host
Rabbit
Reactivity
Human, Monkey, Bat, Guinea pig, Horse (tested or 100% immunogen sequence identity)
Predicted
Bovine, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
TSAP6 / STEAP3 antibody was raised against synthetic peptide located between aa411-460 of human STEAP3 (A8K6E3, NP_878919). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Bat, Horse, Opossum, Guinea pig (100%); Galago, Elephant, Bovine, Rabbit (92%); Mouse, Rat, Hamster, Dog (85%).
Epitope
aa411-460
Specificity
Human STEAP3
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Fetal liver cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal liver cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal liver cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal liver cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal liver cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal liver cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal liver cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal liver cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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