Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Specificity and Use
Recombinant Thymidylate Synthase.
Recognizes a 36kD protein, Thymidylate Synthase (TS). Species cross-reactivity: Human.
Suitable for use in ELISA, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation: 10 ul/mg protein lysate. Western Blot: 1:200-1:400. Immunohistochemistry (frozen and paraffin sections): 1:50-1:100 with incubation for 30-60 min at RT.
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs.