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Anti-Trypsin Inhibitor Antibody LS-C153797

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Trypsin Inhibitor Antibodies

Anti-Trypsin Inhibitor Antibody LS-C59312
Rabbit Polyclonal to Soybean Trypsin Inhibitor
Soybean
Western blot, ELISA
Unconjugated
Anti-Trypsin Inhibitor Antibody LS-C59316
Rabbit Polyclonal to Lima Bean Trypsin Inhibitor
Lima Bean
Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Soybean Trypsin Inhibitor
Soybean
Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Soybean Trypsin Inhibitor
Rabbit
Soybean (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Ion exchange chromatography
Unmodified

Applications

  • Western blot (1:500 - 1:5000)
  • Immunoprecipitation (1:100)
  • ELISA

Specificity and Use

Trypsin Inhibitor [Soy Bean].
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Soy Bean]. Cross reactivity against Trypsin Inhibitor from other sources may occur but have not been specifically determined.
This product has been assayed against 1.0 ug of Trypsin Inhibitor [Soy Bean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:12000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 ug of Trypsin Inhibitor [Soy Bean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:12000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 ug of Trypsin Inhibitor [Soy Bean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:12000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 ug of Trypsin Inhibitor [Soy Bean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:12000 of the reconstitution concentration is suggested for this product.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About Trypsin Inhibitor


Western blot

Western blot
Rabbit anti Soybean Trypsin Inhibitor antibody was used to detect purified Soybean Trypsin Inhibitor. Samples of ~1 and 0.25 ug of purified Soybean Trypsin Inhibitor per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples of protein contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Rabbit anti Soybean Trypsin Inhibitor antibody (200-4679 lot 6594 1:5K in MB-0070, ON 4 C). Primary antibody was detected with Dylight 488 conjugated Streptavidin (S000-41 Lot 21091, 1:5K) and Atto 425 conjugated goat anti rabbit (LS-C153934 lot 26423C 1:10K 1.5 hr RT in MB-070) and imaged on the BioRad VersaDoc imaging system.
 

Requested From: United States
Date Requested: 12/3/2016

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