Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse, Rat (tested or 100% immunogen sequence identity)
Specificity and Use
TRPV4 antibody was raised against synthetic peptide from 1t cytoplasmic domain of mouse TRPV4 conjugated to an immunogenic carrier protein.
Specific for TRPV4.
IHC: Antigen retrieval is essential for use on paraffin sections.
Lyophilized. Centrifuge to remove any insoluble material
100 µl Sterile water
Maintain lyophilized and reconstituted antibodies at -20 °C for long term storage and at 2-8 °C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
The polymodal ion channel transient receptor potential vanilloid 4 modulates calcium flux, spiking rate, and apoptosis of mouse retinal ganglion cells. Ryskamp DA, Witkovsky P, Barabas P, Huang W, Koehler C, Akimov NP, Lee SH, Chauhan S, Xing W, Rentera RC, Liedtke W, Krizaj D. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2011 31:7089-101. (WB, IHC-Fr; Mouse, Human)[Full Text Article]
Neurite outgrowth and synaptophysin expression of postnatal CNS neurons on GaP nanowire arrays in long-term retinal cell culture. Piret G, Perez MT, Prinz CN. Biomaterials. 2013 34:875-87. (ICC, IHC-Fr; Mouse)
Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca2+ potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism.