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Anti-TRPA1 Antibody (N-Terminus) IHC-plus™ LS-B2819

Note: This antibody replaces LS-C69034
Catalog Size Price
LS-B2819-50 50 µl (1 mg/ml) Unavailable

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Anti-TRPA1 Antibody (Internal) IHC-plus™ LS-A9098
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Anti-TRPA1 Antibody (aa1-100) IHC-plus™ LS-B177
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Anti-TRPA1 Antibody (N-Terminus) IHC-plus™ LS-B2819
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Anti-TRPA1 Antibody (aa1050-1120) LS-C94994
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Rabbit Polyclonal (IgG) to Human TRPA1
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated

Details

Human TRPA1
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (2 µg/ml)

Specificity and Use

TRPA1 antibody was raised against synthetic peptide made to a region within the N-terminus (residues 1-100) of the human TRPA1 protein [Swiss-Prot#O75762].
N-Terminus
Recognizes human and mouse ANKTM1.
Immunohistochemistry: LS-B2819 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2819 was determined to be 5 ug/ml. Western Blot: 2 ug/ml. Detects a band at ~110kD.

Packaging

Tris-glycine, 150 mM sodium chloride, 0.05% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About TRPA1

O75762 NM_007332 CAA71610.1

TRPA1 Antibody, ANKTM1 Antibody, TRPA-1 Antibody

Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes.

Immunohistochemistry

Immunohistochemistry
TRPA1 antibody (5 ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human skeletal muscle, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Requested From: 
Date Requested: 10/19/2017

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