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Anti-TRM3 / TARBP1 Antibody (Internal) IHC-plus™ LS-B596

Note: This antibody replaces LS-C19664
Catalog Size Price
LS-B596-50 50 µg (1 mg/ml) Unavailable

Related Products

1 TRM3 / TARBP1 Antibody

Most Popular TRM3 / TARBP1 Antibodies

Anti-TRM3 / TARBP1 Antibody (Internal) IHC-plus™ LS-B596
Rabbit Polyclonal (IgG) to Human TRM3 / TARBP1
Human, Mouse
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-TARBP1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B596 concentration 5 ug/ml.
Anti-TRM3 / TARBP1 Antibody (aa1571-1621) LS-C290291
Rabbit Polyclonal (IgG) to Human TRM3 / TARBP1
Human
Western blot, Immunoprecipitation
Unconjugated
Detection of Human TARBP1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TARBP1 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. TARBP1 was also immunoprecipitated by rabbit anti-TARBP1 antibody BL13044, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

100% Guaranteed Publications (1)
Rabbit Polyclonal (IgG) to Human TRM3 / TARBP1
Human, Mouse
IHC, IHC - Paraffin, Western blot
Unconjugated

Details

Human TRM3 / TARBP1
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot

Specificity and Use

LS-E30297 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B596. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Internal
Immunohistochemistry: LS-B596 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B596 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
For research use only.

Publications (1)

1
Expression of protein TARBP1 in human hepatocellular carcinoma and its prognostic significance. Ye J, Wang J, Tan L, Yang S, Xu L, Wu X, Deng H, Tan H. International journal of clinical and experimental pathology. 2015 8:9089-96. (WB; Human) [PubMed:26464651] [PMC:PMC4583883]

About TRM3 / TARBP1

Q13395 NM_005646 NP_005637.3

TARBP1 Antibody, TAR RNA loop binding protein Antibody, TRM3 Antibody, TRP-185 Antibody, TRP185 Antibody, TAR RNA-binding protein 1 Antibody

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. This element forms a stable stem-loop structure and can be bound by either the protein encoded by this gene or by RNA polymerase II.

Immunohistochemistry

Anti-TARBP1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B596 concentration 5 ug/ml.
Anti-TARBP1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B596 concentration 5 ug/ml.

Immunohistochemistry

Anti-TARBP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B596 concentration 5 ug/ml.
Anti-TARBP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B596 concentration 5 ug/ml.

Western blot

Western blot analysis of TRBP1 in 3T3 cell lysate with TRBP1 antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of TRBP1 in 3T3 cell lysate with TRBP1 antibody at (A) 1 and (B) 2 µg/mL.

Western blot

Western blot analysis of TRBP1 in 3T3 cell lysate with TRBP1 antibody at (A) 1 and (B) 2 ug/ml.
Western blot analysis of TRBP1 in 3T3 cell lysate with TRBP1 antibody at (A) 1 and (B) 2 ug/ml.

Requested From: 
Date Requested: 5/21/2018

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