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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TRIM3 Antibody (aa225‑274) LS‑C31401
TRIM3 antibody LS-C31401 is an unconjugated rabbit polyclonal antibody to TRIM3 from human and rabbit. Validated for IHC and WB.
Catalog
Size
Price
LS-C31401-100
100 µg (1 mg/ml)
Unavailable

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Product Description

TRIM3 antibody LS-C31401 is an unconjugated rabbit polyclonal antibody to TRIM3 from human and rabbit. Validated for IHC and WB.
About TRIM3
TRIM3 is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. O75382 NM_006458 NP_006449.2

TRIM3 Antibody, BERP Antibody, Brain expressed ring finger Antibody, Tripartite motif containing 3 Antibody, Tripartite motif protein TRIM3 Antibody, RING finger protein 97 Antibody, RNF22 Antibody, RNF97 Antibody, HAC1 Antibody, RING finger protein 22 Antibody, Tripartite motif-containing 3 Antibody


Specifications

Target
Human TRIM3
Host
Rabbit
Reactivity
Human, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Zebrafish (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
TRIM3 antibody was raised against synthetic peptide located between aa225-274 of human TRIM3 (Q6ZTE7, NP_006449). Percent identity by BLAST analysis: Human, Chimpanzee, Elephant, Rabbit (100%); Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Horse, Guinea pig, Platypus (92%); Bat, Pig, Zebrafish (91%); Opossum (85%); Turkey, Zebra finch, Chicken, Lizard, Xenopus (83%).
Blocking Peptide
LS-E12339 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C31401. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa225-274
Specificity
Human TRIM3
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
100 µl After adding water, will consist of PBS buffer with 2% sucrose
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

TRIM3 antibody LS-C31401 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TRIM3 antibody LS-C31401 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

TRIM3 antibody LS-C31401 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TRIM3 antibody LS-C31401 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

TRIM3 antibody LS-C31401 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TRIM3 antibody LS-C31401 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

TRIM3 antibody LS-C31401 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TRIM3 antibody LS-C31401 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

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