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TRIM28 / KAP1 Antibody (N‑Terminus) LS‑C109592
KAP1 antibody LS-C109592 is an unconjugated rabbit polyclonal antibody to KAP1 (TRIM28) from human, bovine and monkey. Validated for WB.
Catalog
Size
Price
LS-C109592-50
50 µg
Unavailable

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Product Description

KAP1 antibody LS-C109592 is an unconjugated rabbit polyclonal antibody to KAP1 (TRIM28) from human, bovine and monkey. Validated for WB.
About TRIM28 / KAP1
Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Q13263 NM_005762 NP_005753.1

TRIM28 Antibody, E3 SUMO-protein ligase TRIM28 Antibody, KRIP-1 Antibody, Nuclear corepressor KAP-1 Antibody, KAP-1 Antibody, KAP1 Antibody, KRAB-interacting protein 1 Antibody, TIF1B Antibody, RNF96 Antibody, TIF1-beta Antibody, TIF1beta Antibody, Tripartite motif-containing 28 Antibody, KRAB-associated protein 1 Antibody, RING finger protein 96 Antibody, TF1B Antibody, Tif1beta zinc finger protein Antibody, Tripartite motif containing 28 Antibody


Specifications

Target
Human TRIM28 / KAP1
Host
Rabbit
Reactivity
Human, Monkey, Bovine (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
TRIM28 / KAP1 antibody was raised against synthetic peptide from N-Terminus of human TRIM28 (Q13263, NP_005753). Percent identity by BLAST analysis: Human, Chimpanzee, Marmoset, Bovine (100%); Gibbon, Mouse, Rat (92%).
Blocking Peptide
LS-E10999 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C109592. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human TRIM28
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

TRIM28 antibody LS-C109592 Western blot of MCF7 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TRIM28 antibody LS-C109592 Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TRIM28 antibody LS-C109592 Western blot of MCF7 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TRIM28 antibody LS-C109592 Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TRIM28 antibody LS-C109592 Western blot of MCF7 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TRIM28 antibody LS-C109592 Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TRIM28 antibody LS-C109592 Western blot of MCF7 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TRIM28 antibody LS-C109592 Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/23/2018

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