LSBio
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Biochemicals
  • All Biochemicals

  • Immunohistochemistry
  • Immunohistochemistry Reagents
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Research Areas
  • Cancer Research Resources and Products:
  • Breast Cancer
  • Colorectal Cancer
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Reviews
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Protocols
  • Secure Logins
  • Login to Our Secure ShareFile Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login ▾
Wish List
View Cart

Anti-TRIM28 / KAP1 Antibody (clone 4E6) IHC-plus™ LS-B5439

Note: This antibody replaces LS-C133628
Catalog Size Price
LS-B5439-50 50 µg (0.5 mg/ml) Unavailable

Related Products

57 TRIM28 / KAP1 Antibodies

Most Popular TRIM28 / KAP1 Antibodies

Anti-TRIM28 / KAP1 Antibody (aa823-835) IHC-plus™ LS-B3928
Goat Polyclonal to Human TRIM28 / KAP1
Human, Monkey
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Anti-TRIM28 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3928 concentration 2.5 ug/ml.
Anti-TRIM28 / KAP1 Antibody (clone 4E6) IHC-plus™ LS-B5439
Mouse Monoclonal [clone 4E6] (IgG2b,k) to Human TRIM28 / KAP1
Human, Mouse, Rat
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Anti-TRIM28 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5439 concentration 5 ug/ml.
Anti-TRIM28 / KAP1 Antibody (clone 1D11) IHC-plus™ LS-B5440
Mouse Monoclonal [clone 1D11] (IgG2a,k) to Human TRIM28 / KAP1
Human, Mouse, Rat
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-TRIM28 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5440 concentration 5 ug/ml.
Anti-TRIM28 / KAP1 Antibody (Biotin) LS-C211592
Rabbit Polyclonal (IgG) to Human TRIM28 / KAP1
Human
IHC, Western blot, ELISA
Biotin Conjugated
Western blot of Transcription intermediary factor 1-beta antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 92 kDa. Observed band size: 92 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-TRIM28 / KAP1 Antibody (aa1-50) IHC-plus™ LS-B14470
Rabbit Polyclonal (IgG) to Human TRIM28 / KAP1
Human, Mouse, Bovine, Dog, Horse, Rabbit
IHC, IHC - Paraffin, ICC, Immunofluorescence
Unconjugated
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

100% Guaranteed
Mouse Monoclonal [clone 4E6] (IgG2b,k) to Human TRIM28 / KAP1
Human, Mouse, Rat
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human TRIM28 / KAP1
Mouse
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [4E6]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

TRIM28 / KAP1 antibody was raised against tRIM28 (AAH04978, 379 a.a. ~ 524 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human TRIM28
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Western Blot: Cell lysate, Cell lysate, Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.4
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.

About TRIM28 / KAP1

Q13263 NM_005762 NP_005753.1

TRIM28 Antibody, E3 SUMO-protein ligase TRIM28 Antibody, KRIP-1 Antibody, Nuclear corepressor KAP-1 Antibody, KAP-1 Antibody, KAP1 Antibody, KRAB-interacting protein 1 Antibody, TIF1B Antibody, RNF96 Antibody, TIF1-beta Antibody, TIF1beta Antibody, Tripartite motif-containing 28 Antibody, KRAB-associated protein 1 Antibody, RING finger protein 96 Antibody, TF1B Antibody, Tif1beta zinc finger protein Antibody, Tripartite motif containing 28 Antibody

Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes.

Immunohistochemistry

Anti-TRIM28 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5439 concentration 5 ug/ml.
Anti-TRIM28 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5439 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to TRIM28 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to TRIM28 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

TRIM28 monoclonal antibody, clone 4E6. Western blot of TRIM28 expression in PC-12.
TRIM28 monoclonal antibody, clone 4E6. Western blot of TRIM28 expression in PC-12.

Western blot

Western blot of TRIM28 expression in transfected 293T cell line by TRIM28 monoclonal antibody, clone 4E6.
Western blot of TRIM28 expression in transfected 293T cell line by TRIM28 monoclonal antibody, clone 4E6.

RNA interference

Western blot of TRIM28 over-expressed 293 cell line, cotransfected with TRIM28 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM28 monoclonal antibody clone 4E6. GAPDH ( 36.1 kD ) used as control.
Western blot of TRIM28 over-expressed 293 cell line, cotransfected with TRIM28 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM28 monoclonal antibody clone 4E6. GAPDH ( 36.1 kD ) used as control.

Requested From: 
Date Requested: 5/26/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number