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Antibodies > TRIM28 / KAP1 > LS-C160184
 
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Catalog Number Size Price
LS-C160184-400 400 µl $393 
TRIM28 / KAP1 Antibody - TRIM28 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human testis tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
TRIM28 / KAP1 Antibody - Fluorescent confocal image of HeLa cell stained with TRIM28 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TRIM28 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TRIM28 immunoreactivity is localized to Nucleus significantly.
TRIM28 / KAP1 Antibody - TRIM28 Antibody western blot of K562 cell line lysates (35 ug/lane). The TRIM28 antibody detected the TRIM28 protein (arrow).
TRIM28 / KAP1 Antibody - TRIM28 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human testis tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
TRIM28 / KAP1 Antibody - Fluorescent confocal image of HeLa cell stained with TRIM28 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TRIM28 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TRIM28 immunoreactivity is localized to Nucleus significantly.
TRIM28 / KAP1 Antibody - TRIM28 Antibody western blot of K562 cell line lysates (35 ug/lane). The TRIM28 antibody detected the TRIM28 protein (arrow).
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Polyclonal Rabbit anti‑Human TRIM28 / KAP1 Antibody (aa178‑207, IHC, IF, WB) LS‑C160184

Polyclonal Rabbit anti‑Human TRIM28 / KAP1 Antibody (aa178‑207, IHC, IF, WB) LS‑C160184

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Antibody:
TRIM28 / KAP1 Rabbit anti-Human Polyclonal (aa178-207) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C160184-400
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
TRIM28 / KAP1 Rabbit anti-Human Polyclonal (aa178-207) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
KAP1 antibody LS-C160184 is an unconjugated rabbit polyclonal antibody to human KAP1 (TRIM28) (aa178-207). Validated for IF, IHC and WB.
Target
Human TRIM28 / KAP1
Synonyms
TRIM28 | E3 SUMO-protein ligase TRIM28 | KRIP-1 | Nuclear corepressor KAP-1 | KAP-1 | KAP1 | KRAB-interacting protein 1 | TIF1B | RNF96 | TIF1-beta | TIF1beta | Tripartite motif-containing 28 | KRAB-associated protein 1 | RING finger protein 96 | TF1B | Tif1beta zinc finger protein | Tripartite motif containing 28
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa178-207
Specificity
This TRIM28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-207 amino acids from the N-terminal region of human TRIM28.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TRIM28 / KAP1
Q13263 NM_005762 NP_005753.1

Publications (0)

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Featured Products

TRIM28 / KAP1 Antibody - Anti-TRIM28 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ TRIM28 / KAP1 Antibody (clone 4E6) LS‑B5439
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA, RNA interference
TRIM28 / KAP1 Antibody - IHC of paraffin-embedded human Spleen using anti-KAP1 / TIF1 beta diluted 1/500-1/1000.
TRIM28 / KAP1 Antibody LS‑C393503
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
TRIM28 / KAP1 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ TRIM28 / KAP1 Antibody (aa1‑50) LS‑B14470
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence
TRIM28 / KAP1 Antibody - Detection of Human KAP-1 by Immunohistochemistry and Immunocytochemistry. Sample: FFPE section of human prostate carcinoma (left) and formaldelyde-fixed HeLa cells (right). Antibody: Affinity purified rabbit anti-KAP-1 used at a dilution of 1:1000 (1μg/ml). Detection: DAB (left) and green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated used at a dilution of 1:100 (right).
TRIM28 / KAP1 Antibody (aa1‑50) LS‑C287177
Species: Human, Mouse
Applications: IHC, ICC, Western blot, Immunoprecipitation
TRIM28 / KAP1 Antibody - Western blot of Transcription intermediary factor 1-beta antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 92 kDa. Observed band size: 92 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
TRIM28 / KAP1 Antibody (Biotin) LS‑C211592
Species: Human
Applications: IHC, Western blot, ELISA
TRIM28 / KAP1 Protein - Purified recombinant protein TRIM28 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Human TRIM28 / KAP1 Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G68131
Source: Human
Tag: Myc-DDK (Flag)
See all TRIM28 / KAP1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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