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Anti-TRIM21 / RO52 Antibody IHC-plus™ LS-B3096

Note: This antibody replaces LS-C10349

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TRIM21 / RO52 Antibodies

Anti-TRIM21 / RO52 Antibody (aa51-100) LS-C80190
Rabbit Polyclonal (IgG) to Human TRIM21 / RO52
Human, Monkey, Mouse, Rat, Pig
Western blot
Unconjugated
Western blot Image
Anti-TRIM21 / RO52 Antibody LS-C132187
Human Polyclonal to Human TRIM21 / RO52
Human
ELISA, Immunodiffusion
Unconjugated
Anti-TRIM21 / RO52 Antibody (aa195-293, clone TS106) LS-C191493
Mouse Monoclonal [clone TS106] (IgG1) to Human TRIM21 / RO52
Human
ICC
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human TRIM21 / RO52
Human, Horse
IHC - Paraffin, ELISA
Unconjugated

Details

Human TRIM21 / RO52
Rabbit
Human, Horse (tested or 100% immunogen sequence identity)
Monkey, Bovine, Dog (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • ELISA

Specificity and Use

TRIM21 / RO52 antibody was raised against synthetic peptide, WNKQKYEAGTYPQTPL of human SSA1 (KLH). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Horse (100%); Gorilla, Marmoset, Dog, Bovine (94%); Pig (88%); Panda, Bat (81%).
Recognizes human SSA1. Species cross-reactivity: rodent.
Immunohistochemistry: LS-B3096 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3096 was determined to be 10 ug/ml.

Packaging

PBS, pH 7.2, sodium azide, before the addition of glycerol to 40%
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About TRIM21 / RO52

P19474 NM_003141 NP_003132.2

TRIM21 Antibody, 52 kDa Ro protein Antibody, RING finger protein 81 Antibody, RO52 Antibody, Ro(SS-A) Antibody, SSA1 Antibody, Tripartite motif-containing 21 Antibody, RNF81 Antibody, SSA Antibody, Sicca syndrome antigen A Antibody, SS-A Antibody, Tripartite motif containing 21 Antibody

E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes.

Immunohistochemistry

Immunohistochemistry
Anti-TRIM21 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3096 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-TRIM21 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3096 concentration 10 ug/ml.

Requested From: United States
Date Requested: 12/9/2016

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