Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Specificity and Use
TRIM21 / RO52 antibody was raised against synthetic peptide from C-Terminus of human TRIM21 (P19474, NP_003132). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Gibbon, Monkey, Horse (92%); Marmoset, Pig (84%).
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Lyophilized from PBS with 2% sucrose
100 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
TRIM21 Antibody, 52 kDa Ro protein Antibody, RING finger protein 81 Antibody, RO52 Antibody, Ro(SS-A) Antibody, SSA1 Antibody, Tripartite motif-containing 21 Antibody, RNF81 Antibody, SSA Antibody, Sicca syndrome antigen A Antibody, SS-A Antibody, Tripartite motif containing 21 Antibody
E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes.