LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-TRIM21 / RO52 Antibody (C-Terminus) LS-C80191

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TRIM21 / RO52 Antibodies

Anti-TRIM21 / RO52 Antibody (aa51-100) LS-C80190
Rabbit Polyclonal (IgG) to Human TRIM21 / RO52
Human, Monkey, Mouse, Rat, Pig
Western blot
Unconjugated
Western blot Image
Anti-TRIM21 / RO52 Antibody LS-C132187
Human Polyclonal to Human TRIM21 / RO52
Human
ELISA, Immunodiffusion
Unconjugated
Anti-TRIM21 / RO52 Antibody (aa195-293, clone TS106) LS-C191493
Mouse Monoclonal [clone TS106] (IgG1) to Human TRIM21 / RO52
Human
ICC
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human TRIM21 / RO52
Human, Guinea pig, Pig
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human TRIM21 / RO52
Rabbit
Human, Guinea pig, Pig (tested or 100% immunogen sequence identity)
Monkey, Horse (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Protein A purified
Unmodified

Applications

  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

TRIM21 / RO52 antibody was raised against synthetic peptide from C-Terminus of human TRIM21 (P19474, NP_003132). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Gibbon, Monkey, Horse (92%); Marmoset, Pig (84%).
C-Terminus
Human TRIM21
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
100 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About TRIM21 / RO52

P19474 NM_003141 NP_003132.2

TRIM21 Antibody, 52 kDa Ro protein Antibody, RING finger protein 81 Antibody, RO52 Antibody, Ro(SS-A) Antibody, SSA1 Antibody, Tripartite motif-containing 21 Antibody, RNF81 Antibody, SSA Antibody, Sicca syndrome antigen A Antibody, SS-A Antibody, Tripartite motif containing 21 Antibody

E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes.

Western blot

Western blot
Transfected 293T cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/4/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number