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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TRIM21 / RO52 Antibody (aa386‑398) IHC‑plus™ LS‑B2588
Note: This antibody replaces LS-C55529
RO52 antibody LS-B2588 is an unconjugated goat polyclonal antibody to RO52 (TRIM21) from human and monkey. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2588-50
50 µg (0.5 mg/ml)
Unavailable

Popular TRIM21 / RO52 Products

Transfected 293T cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Applications: Western blot
Transfected 293T cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Guinea pig, Pig
Applications: Western blot
Anti-TRIM21 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3096 concentration 10 ug/ml.
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Applications: IHC, IHC - Paraffin, ELISA

Product Description

RO52 antibody LS-B2588 is an unconjugated goat polyclonal antibody to RO52 (TRIM21) from human and monkey. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
About TRIM21 / RO52
E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. P19474 NM_003141 NP_003132.2

TRIM21 Antibody, 52 kDa Ro protein Antibody, RING finger protein 81 Antibody, RO52 Antibody, Ro(SS-A) Antibody, SSA1 Antibody, Tripartite motif-containing 21 Antibody, RNF81 Antibody, SSA Antibody, Sicca syndrome antigen A Antibody, SS-A Antibody, Tripartite motif containing 21 Antibody


Specifications

Target
Human TRIM21 / RO52
Host
Goat
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
TRIM21 / RO52 antibody was raised against synthetic peptide CPLNIGSQGSTDY from the C-terminus of human TRIM21 (NP_003132.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset (85%).
Blocking Peptide
LS-E25520 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2588. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa386-398
Specificity
Human TRIM21.
Usage
Immunohistochemistry: LS-B2588 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2588 was determined to be 5 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-TRIM21 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2588 concentration 5 ug/ml.
Anti-TRIM21 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2588 concentration 5 ug/ml.

Western blot

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Detect
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Detect

Immunohistochemistry

Anti-TRIM21 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2588 concentration 5 ug/ml.
Anti-TRIM21 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2588 concentration 5 ug/ml.

Western blot

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Detect
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Detect

Immunohistochemistry

Anti-TRIM21 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2588 concentration 5 ug/ml.
Anti-TRIM21 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2588 concentration 5 ug/ml.

Western blot

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Detect
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Detect

Immunohistochemistry

Anti-TRIM21 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2588 concentration 5 ug/ml.
Anti-TRIM21 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2588 concentration 5 ug/ml.

Western blot

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Detect
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Detect


Requested From: 
Date Requested: 6/17/2018

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