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Anti-TRIM14 Antibody (aa116-165) IHC-plus™ LS-B1102

Note: This antibody replaces LS-C29995

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TRIM14 Antibodies

Anti-TRIM14 Antibody (aa116-165) LS-C31636
Rabbit Polyclonal (IgG) to Human TRIM14
Human, Bat, Dog, Rabbit
Western blot
Unconjugated
Western blot Image
Anti-TRIM14 Antibody (aa116-165) IHC-plus™ LS-B1102
Rabbit Polyclonal to Human TRIM14
Human, Bat, Dog, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-TRIM14 Antibody LS-C89715
Rabbit Polyclonal to Human TRIM14
Human
Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human TRIM14
Human, Bat, Dog, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human TRIM14
Rabbit
Human, Bat, Dog, Rabbit (tested or 100% immunogen sequence identity)
Monkey, Mouse, Rat, Bovine, Horse, Pig (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

TRIM14 antibody was raised against synthetic peptide located between aa116-165 of human TRIM14 (Q5TBQ8). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Bat (100%); Monkey, Marmoset, Mouse, Rat, Bovine, Horse, Pig (92%); Galago, Dog, Rabbit, Guinea pig (85%).
LS-E12364 - Lyophilized - 100 µg - $145.00
aa116-165
Human TRIM14
Immunohistochemistry: LS-B1102 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1102 was determined to be 2.5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl After adding water, will consist of PBS buffer with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About TRIM14

Q14142 NM_014788 NP_055603.2

TRIM14 Antibody, KIAA0129 Antibody, Tripartite motif containing 14 Antibody, Tripartite motif-containing 14 Antibody

TRIM14 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies and its function has not been determined. Alternative splicing results in multiple transcript variants.

Immunohistochemistry

Immunohistochemistry
Anti-TRIM14 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1102 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
TRIM14 antibody LS-B1102 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/11/2016

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