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Anti-TRIM13 Antibody (aa165-214) IHC-plus™ LS-B1007

Note: This antibody replaces LS-C29949
Catalog Size Price
LS-B1007-50 50 µg Unavailable

Related Products

29 TRIM13 Antibodies

Most Popular TRIM13 Antibodies

Anti-TRIM13 Antibody (aa165-214) IHC-plus™ LS-B1007
Rabbit Polyclonal to Human TRIM13
Human, Monkey, Rat, Bat, Bovine, Guinea pig, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-TRIM13 Antibody LS-C346104
Rabbit Polyclonal (IgG) to Human TRIM13
Human
IHC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescence Image
Anti-TRIM13 Antibody (aa165-214, Biotin) LS-C430632
Rabbit Polyclonal to Human TRIM13
Human, Chimpanzee, Orangutan, Gibbon, Monkey, Rat, Bat, Bovine, Guinea pig, Hamster, Horse, Pig, Rabbit
IHC, IHC - Paraffin, Western blot
Biotin Conjugated
Immunohistochemistry Image
Anti-TRIM13 Antibody (aa165-214, FITC) LS-C430633
Rabbit Polyclonal to Human TRIM13
Human, Chimpanzee, Orangutan, Gibbon, Monkey, Rat, Bat, Bovine, Guinea pig, Hamster, Horse, Pig, Rabbit
IHC, IHC - Paraffin, Western blot
FITC Conjugated
Immunohistochemistry Image
Anti-TRIM13 Antibody (aa165-214, HRP) LS-C430634
Rabbit Polyclonal to Human TRIM13
Human, Chimpanzee, Orangutan, Gibbon, Monkey, Rat, Bat, Bovine, Guinea pig, Hamster, Horse, Pig, Rabbit
IHC, IHC - Paraffin, Western blot
HRP Conjugated
Immunohistochemistry Image

100% Guaranteed
Rabbit Polyclonal to Human TRIM13
Human, Monkey, Rat, Bat, Bovine, Guinea pig, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human TRIM13
Rabbit
Human, Monkey, Rat, Bat, Bovine, Guinea pig, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Mouse, Dog (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.13 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

TRIM13 antibody was raised against synthetic peptide located between aa165-214 of human TRIM13 (Q5UBW0, NP_001007279). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Rat, Hamster, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus (100%); Mouse, Dog (92%); Turkey, Zebra finch, Chicken (85%).
LS-E12076 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B1007. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa165-214
Human TRIM13 / RFP2
Immunohistochemistry: LS-B1007 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1007 was determined to be 5 ug/ml. Antibody was tested in a peptide-based ELISA.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl After adding water, will consist of PBS buffer with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About TRIM13

O60858 NM_005798 NP_005789.2

TRIM13 Antibody, DLEU5 Antibody, CLL-associated RING finger Antibody, LEU5 Antibody, Leukemia-associated protein 5 Antibody, RING finger protein 77 Antibody, Tripartite motif protein 13 Antibody, Putative tumor suppressor RFP2 Antibody, RFP2 Antibody, CAR Antibody, Ret finger protein 2 Antibody, RNF77 Antibody, Tripartite motif containing 13 Antibody, Tripartite motif-containing 13 Antibody

E3 ubiquitin ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation.

Immunohistochemistry

Immunohistochemistry
Anti-RFP2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1007 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
TRIM13 / RFP2 antibody LS-B1007 Western blot of Fetal lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Fetal Lung Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 10/21/2017

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