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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TRB3 / TRIB3 Antibody (C‑Terminus) IHC‑plus™ LS‑A3372
Note: This antibody replaces LS-C89692
TRIB3 antibody LS-A3372 is an unconjugated rabbit polyclonal antibody to human TRIB3 (TRB3). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A3372-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment

Popular TRB3 / TRIB3 Products

Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Antibody
Species: Mouse
Applications: Western blot
Anti-TRIB3 antibody LS-A2976 IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin
IHC of paraffin-embedded Human Kidney tissue using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

TRIB3 antibody LS-A3372 is an unconjugated rabbit polyclonal antibody to human TRIB3 (TRB3). Validated for IHC. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human TRB3 / TRIB3
Synonyms
TRIB3, C20orf97, NIPK, SINK, TRB-3, TRB3, DJ1103G7.3, SKIP3, Tribbles homolog 3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bovine, Dog, Pig, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
TRB3 / TRIB3 antibody was raised against synthetic 16 amino acid peptide from near C-terminus of human TRIB3. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%); Gorilla, Marmoset, Panda, Dog, Bovine, Rabbit, Pig (94%); Elephant (88%); Mouse, Horse, Opossum (81%).
Blocking Peptide
LS-E28733 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A3372. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human TRIB3. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except TRIB2 (75%).
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About TRB3 / TRIB3
Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. Q96RU7 NM_021158 NP_066981.2


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Images

Immunohistochemistry

Anti-TRIB3 antibody LS-A3372 IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-TRIB3 antibody LS-A3372 IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-TRIB3 antibody LS-A3372 IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-TRIB3 antibody LS-A3372 IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-TRIB3 antibody LS-A3372 IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-TRIB3 antibody LS-A3372 IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-TRIB3 antibody LS-A3372 IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-TRIB3 antibody LS-A3372 IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: United States
Date Requested: 10/23/2018

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