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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TRAT1 / TRIM Antibody (aa29‑186, clone TRIM‑04, PE) LS‑C46497
TRIM antibody LS-C46497 is a PE-conjugated mouse monoclonal antibody to human TRIM (TRAT1). Validated for Flow, IP and WB.
Catalog
Size
Price
LS-C46497-100
100 µg
$375

Popular TRAT1 / TRIM Products

Extracts from peripheral blood mononuclear cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse monoclonal antibody against TRIM. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a ch
Species: Human, Mouse
Applications: Immunofluorescence, Western blot, Flow Cytometry
Antibody
Species: Human
Applications: Western blot, Flow Cytometry, ELISA
Western blot of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by  mAb TRIM-04 and goat anti-mouse IgG (H+L)-HRP conjugate.
Species: Human
Applications: Western blot, Immunoprecipitation, Flow Cytometry
Antibody
Species: Human
Applications: Western blot, Flow Cytometry, ELISA
Immunohistochemistry of paraffin-embedded human thymus tissue using antibody at dilution of 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA

Product Description

TRIM antibody LS-C46497 is a PE-conjugated mouse monoclonal antibody to human TRIM (TRAT1). Validated for Flow, IP and WB.

Specifications

Target
Human TRAT1 / TRIM
Synonyms
TRAT1, Pp29/30, TCRIM, TRIM, HSPC062
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [TRIM-04]
Conjugations
Phycoerythrin. Also available Unconjugated.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry (1:50)
  • (applications tested for the base form of this product only)
Immunogen
TRAT1 / TRIM antibody was raised against recombinant intracellular domain (aa 29-186) of human TRIM.
Epitope
aa29-186
Specificity
The antibody TRIM-04 reacts with T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.
Usage
The reagent is designed for Flow Cytometry analysis (see application note on<u> www.exbio.cz</u>). Suggested working dilution is 1:50. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 15 mM Sodium Azide
Storage
Store in the dark at 2°C to 8°C. Do not freeze. Protect from light.
Restrictions
For research use only.
About TRAT1 / TRIM
Stabilizes the TCR (T-cell antigen receptor)/CD3 complex at the surface of T-cells. Q6PIZ9 NM_016388 NP_057472.2


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Images

Flow Cytometry

Intracellular staining of human peripheral blood with  mAb TRIM-04 PE.
Intracellular staining of human peripheral blood with  mAb TRIM-04 PE.

Flow Cytometry

Intracellular staining of human peripheral blood with  mAb TRIM-04 PE.
Intracellular staining of human peripheral blood with  mAb TRIM-04 PE.

Flow Cytometry

Intracellular staining of human peripheral blood with  mAb TRIM-04 PE.
Intracellular staining of human peripheral blood with  mAb TRIM-04 PE.

Flow Cytometry

Intracellular staining of human peripheral blood with  mAb TRIM-04 PE.
Intracellular staining of human peripheral blood with  mAb TRIM-04 PE.

Requested From: United States
Date Requested: 10/23/2018

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