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Antibodies > TRAF3IP3 > LS-C353565
 
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Catalog Number Size Price
LS-C353565-100 100 µl (1 mg/ml) $299 
TRAF3IP3 Antibody - Immunohistochemical analysis of TRAF3IP3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
TRAF3IP3 Antibody - Western blot analysis of TRAF3IP3 expression in MDAMB453 (A); Jurkat (B); HeLa (C) whole cell lysates.
TRAF3IP3 Antibody - Immunoprecipitation of TRAF3IP3 from 0.5mg Mouse cortex tissue lysate using 5ug of Anti-TRAF3IP3 Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min Mouse cortex extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70 C; 10ul of each sample was separated on a SDS PAGE gel transferred to a nitrocellulose membrane blocked with 5% BSA and probed with Anti-TRAF3IP3 Antibody.
TRAF3IP3 Antibody - Immunohistochemical analysis of TRAF3IP3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
TRAF3IP3 Antibody - Western blot analysis of TRAF3IP3 expression in MDAMB453 (A); Jurkat (B); HeLa (C) whole cell lysates.
TRAF3IP3 Antibody - Immunoprecipitation of TRAF3IP3 from 0.5mg Mouse cortex tissue lysate using 5ug of Anti-TRAF3IP3 Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min Mouse cortex extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70 C; 10ul of each sample was separated on a SDS PAGE gel transferred to a nitrocellulose membrane blocked with 5% BSA and probed with Anti-TRAF3IP3 Antibody.
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Polyclonal Rabbit anti‑Human TRAF3IP3 Antibody (Internal, IHC, WB) LS‑C353565

Polyclonal Rabbit anti‑Human TRAF3IP3 Antibody (Internal, IHC, WB) LS‑C353565

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Antibody:
TRAF3IP3 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Bovine
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C353565-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
TRAF3IP3 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Bovine
Format:
Unconjugated, Unmodified

Specifications

Description
TRAF3IP3 antibody LS-C353565 is an unconjugated rabbit polyclonal antibody to TRAF3IP3 (Internal) from human. It is reactive with human and bovine. Validated for IHC, IP and WB.
Target
Human TRAF3IP3
Synonyms
TRAF3IP3 | DJ434O14.3 | TRAF3 interacting protein 3 | TRAF3-interacting protein 3 | T3JAM
Host
Rabbit
Reactivity
Human, Bovine (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human TRAF3IP3.
Epitope
Internal
Specificity
Recognizes endogenous levels of TRAF3IP3 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TRAF3IP3
Q9Y228 NM_025228 NP_079504.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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