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Anti-TPPP Antibody (aa21-35) IHC-plus™ LS-B9213

Note: This antibody replaces LS-C139578

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TPPP Antibodies

Anti-TPPP Antibody (aa21-35) IHC-plus™ LS-B9213
Goat Polyclonal to Human TPPP
Human, Monkey
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-TPPP Antibody (aa46-75, Biotin) LS-C272032
Rabbit Polyclonal (IgG) to Human TPPP
Human
Western blot, ELISA
Biotin Conjugated
Anti-TPPP Antibody (aa46-75, Azide-free, HRP) LS-C272035
Rabbit Polyclonal (IgG) to Human TPPP
Human
Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human TPPP
Human, Monkey
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human TPPP
Goat
Human, Monkey (tested or 100% immunogen sequence identity)
Dog (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.00 - 0.01 µg/ml)
  • ELISA (1:32000)

Specificity and Use

TPPP antibody was raised against synthetic peptide C-DPSKDRAAKRLSLES from an internal region of human TPPP (NP_008961.1). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%); Dog (93%); Panda, Pig (87%); Mouse, Rat, Hamster, Bat (80%).
LS-E27260 - Lyophilized - 100 µg - $145.00
aa21-35
Human TPPP.
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 26kD band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7kD according to NP_008961.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Acevedo et al, Exp Cell Res. 2007 Dec 10;313(20):4091-106; PMID: 18028908). Recommended concentration: 0.001-0.01 ug/ml. An additional band of unknown identity was also consistently observed at 17kD. This band was successfully blocked by incubation with the immunizing peptide.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About TPPP

O94811 NM_007030 NP_008961.1

TPPP Antibody, 25 kDa brain-specific protein Antibody, p25-alpha Antibody, p25 Antibody, p25alpha Antibody, p24 Antibody, TPPP1 Antibody

May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown.

Immunohistochemistry

Immunohistochemistry
Anti-TPPP antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

Western blot
TPPP antibody (0.001 ug/ml) staining of Human Cerebellum lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 12/6/2016

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