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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TPM4 Antibody (clone 4E4‑1D2) LS‑C133492 Best Seller
TPM4 antibody LS-C133492 is an unconjugated mouse monoclonal antibody to human TPM4. Validated for ELISA, IF, IHC and WB.
Catalog
Size
Price
LS-C133492-100
100 µg (0.5 mg/ml)
Unavailable

Popular TPM4 Products

Immunohistochemistry Image
Species: Human, Mouse, Rat
Applications: IHC, ELISA
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Applications: IHC, ICC, Western blot
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
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Species: Human
Applications: IHC, Western blot, Immunoprecipitation, ELISA

Product Description

TPM4 antibody LS-C133492 is an unconjugated mouse monoclonal antibody to human TPM4. Validated for ELISA, IF, IHC and WB.
About TPM4
TPM4 is a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. P67936 NM_003290 NP_003281.1

TPM4 Antibody, Tropomyosin alpha-4 chain Antibody, Tropomyosin 4 Antibody, TM30p1 Antibody, Tropomyosin-4 Antibody


Specifications

Target
Human TPM4
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [4E4-1D2]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
TPM4 antibody was raised against tPM4 (AAH37576, 1 a.a. ~ 249 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human TPM4
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Immunoperoxidase of monoclonal antibody to TPM4 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. [antibody concentration 2 ug/ml].
Immunoperoxidase of monoclonal antibody to TPM4 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. [antibody concentration 2 ug/ml].

Immunofluorescence

Immunofluorescence of monoclonal antibody to TPM4 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to TPM4 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

TPM4 monoclonal antibody (M01), clone 4E4-1D2 Western blot of TPM4 expression in HeLa.
TPM4 monoclonal antibody (M01), clone 4E4-1D2 Western blot of TPM4 expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged TPM4 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged TPM4 is approximately 1 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to TPM4 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. [antibody concentration 2 ug/ml].
Immunoperoxidase of monoclonal antibody to TPM4 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. [antibody concentration 2 ug/ml].

Immunofluorescence

Immunofluorescence of monoclonal antibody to TPM4 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to TPM4 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

TPM4 monoclonal antibody (M01), clone 4E4-1D2 Western blot of TPM4 expression in HeLa.
TPM4 monoclonal antibody (M01), clone 4E4-1D2 Western blot of TPM4 expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged TPM4 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged TPM4 is approximately 1 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to TPM4 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. [antibody concentration 2 ug/ml].
Immunoperoxidase of monoclonal antibody to TPM4 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. [antibody concentration 2 ug/ml].

Immunofluorescence

Immunofluorescence of monoclonal antibody to TPM4 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to TPM4 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

TPM4 monoclonal antibody (M01), clone 4E4-1D2 Western blot of TPM4 expression in HeLa.
TPM4 monoclonal antibody (M01), clone 4E4-1D2 Western blot of TPM4 expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged TPM4 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged TPM4 is approximately 1 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to TPM4 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. [antibody concentration 2 ug/ml].
Immunoperoxidase of monoclonal antibody to TPM4 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. [antibody concentration 2 ug/ml].

Immunofluorescence

Immunofluorescence of monoclonal antibody to TPM4 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to TPM4 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

TPM4 monoclonal antibody (M01), clone 4E4-1D2 Western blot of TPM4 expression in HeLa.
TPM4 monoclonal antibody (M01), clone 4E4-1D2 Western blot of TPM4 expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged TPM4 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged TPM4 is approximately 1 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/23/2018

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