P73 antibody LS-C17142 is an unconjugated rabbit polyclonal antibody to human p73 (TP73). Validated for IHC, IP and WB.
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- Western blot (1 µg/ml)
- (applications tested for the base form of this product only)
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TP73 / p73 antibody was raised against gST fusion protein that contains aa450-636 of the p73 protein.
Recognizes amino acids 450-636 of human p73 protein. This region is common to both p73 alpha and p73 beta isoforms, hence both isoforms of the p73 protein will be detected.
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1 ug/ml of the antibody will detect p73 in 30 ug of total cell lysate from overexpressing cell lines. Higher levels of antibody may be necessary when using native tissue sources. Immunohistochemistry: It may be necessary to incorporate a signal amplification technique in conjunction with standard procedures.
PBS, pH 7.4, 0.05% Sodium Azide, 40% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.