Human TP53BP1 / 53BP1

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein G purified

Unmodified

Mix of three GST fusion proteins corresponding to residues 1-337, 338-671, and 1331-1664, respectively, of human 53BP1.

aa1-337

Recognizes human 53BP1, Mr ~250kD.

• ICC
• Western blot
• Immunoprecipitation

Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot Analysis: 1-4 ug/ml of this lot detected 53BP1 in lysates from HeLa cells. Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate 53BP1 from HeLa cell lysate. Immunocytochemistry: Has been reported by an independent laboratory to show positive immunostaining for 53BP1 in HeLa cells fixed with 3% paraformaldehyde and permeabilized in 0.5% Triton X-100.

0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q12888 NCBI: NM_005657 NP_005648.1