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Catalog Number Size Price
LS-B9038-200 200 µl $460 
TP53 / p53 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
TP53 / p53 Antibody - Fluorescent confocal image of U251 cell stained with p53 Antibody (S315). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with p53 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). p53 immunoreactivity is localized to Nucleus significantly.
TP53 / p53 Antibody - p53 Antibody (S315) western blot of Daudi cell line lysates (35 ug/lane). The p53 antibody detected the p53 protein (arrow).
TP53 / p53 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
TP53 / p53 Antibody - Fluorescent confocal image of U251 cell stained with p53 Antibody (S315). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with p53 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). p53 immunoreactivity is localized to Nucleus significantly.
TP53 / p53 Antibody - p53 Antibody (S315) western blot of Daudi cell line lysates (35 ug/lane). The p53 antibody detected the p53 protein (arrow).
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IHC‑plus™ Polyclonal Rabbit anti‑Human TP53 / p53 Antibody (Ser315, IHC, IF, WB) LS‑B9038

IHC‑plus™ Polyclonal Rabbit anti‑Human TP53 / p53 Antibody (Ser315, IHC, IF, WB) LS‑B9038

Note: This antibody replaces LS-C99925
Antibody:
TP53 / p53 Rabbit anti-Human Polyclonal (Ser315) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B9038-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TP53 / p53 Rabbit anti-Human Polyclonal (Ser315) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
P53 antibody LS-B9038 is an unconjugated rabbit polyclonal antibody to human p53 (TP53) (Ser315). Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TP53 / p53
Synonyms
TP53 | BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
Ser315
Specificity
This p53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-322 amino acids from human p53.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS with 0.09% (w/v) sodium azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TP53 / p53
P04637 NM_000546 NP_000537.3

Validation

TP53 / p53 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ TP53 / p53 Antibody (Ser315) for IHC, IF/Immunofluorescence, WB/Western LS-B9038 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/20/2024