Human TP53 / p53

Mouse

Human, Monkey, Bovine (tested or 100% immunogen sequence identity)

IgG2b,k Monoclonal

DO-7

Unconjugated

Protein G purified

Ready-to-Use. Also available Unmodified or Azide-free, BSA-free.

Recombinant human wild type p53 expressed in E. coli.

Human TP53 / p53

• IHC
• IHC - Paraffin (1:1)
• Immunofluorescence
• Western blot
• Immunoprecipitation
• Flow Cytometry
• ELISA

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This antibody is specific for a 53kDa protein, which is identified as p53 suppressor gene product. The large number of prolines in its amino acid sequence causes p53 to migrate slowly in SDS-PAGE, resulting in the amino acid content-estimated 43 kDa protein appearing larger than expected. DO-7 reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intra-tubular germ cell neoplasia. ELISA: Order BSA-free antibody format for coating.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P04637 NCBI: NM_000546 NP_000537.3