LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-TP53 / p53 Antibody (clone BP53-12) IHC-plus™ LS-B431

Note: This antibody replaces LS-C19012

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TP53 / p53 Antibodies

Anti-TP53 / p53 Antibody (clone BP53-12) IHC-plus™ LS-B431
Mouse Monoclonal [clone BP53-12] (IgG2a,k) to Human TP53 / p53
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-TP53 / p53 Antibody (clone PAb240, Azide-free) LS-C87442
Mouse Monoclonal [clone PAb240] (IgG1) to Mouse TP53 / p53
Mouse, Human
IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated, Azide-free
Immunohistochemistry Image
Anti-TP53 / p53 Antibody (clone 6F2D3) LS-C203312
Mouse Monoclonal [clone 6F2D3] (IgG1) to Human TP53 / p53
Human
Western blot, Flow Cytometry, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed Publications Publications (3)
Mouse Monoclonal [clone BP53-12] (IgG2a,k) to Human TP53 / p53
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human TP53 / p53
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [BP53-12]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • ICC
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
  • ELISA (1:2000 - 1:10000)

Specificity and Use

TP53 / p53 antibody was raised against recombinant human TP53 / p53.
Recombinant human p53 protein. Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma P3-X63/AG8.653 cells using conventional hybridoma technology.
Immunohistochemistry: LS-B431 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B431 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.5 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (3)

1
Cancer. p53, guardian of the genome. Lane DP. Nature. 1992 358:15-6. [PubMed:1614522]
2
p53 mutations in human cancers. Hollstein M, Sidransky D, Vogelstein B, Harris CC. Science (New York, N.Y.). 1991 253:49-53. [PubMed:1905840]
3
Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens. Gurney EG, Harrison RO, Fenno J. Journal of virology. 1980 34:752-63. [PubMed:6155477] [PMC:PMC288763]

About TP53 / p53

P04637 NM_000546 NP_000537.3

TP53 Antibody, BCC7 Antibody, Cellular tumor antigen p53 Antibody, p53 Antibody, TRP53 Antibody, p53 tumor suppressor Antibody, Phosphoprotein p53 Antibody, Antigen NY-CO-13 Antibody, Tumor protein p53 Antibody, Tumor suppressor p53 Antibody, LFS1 Antibody

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases.

Immunohistochemistry

Immunohistochemistry
Anti-TP53 / p53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B431 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
p53 Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy of HeLa cells using anti-p53. Protein A purified Mab anti-p53 was used at a 1:100 dilution in 10% normal goat serum in PBS and reacted overnight at 4° C. After washes cells were incubated with a 1:500 dilution of Alexa Fluor594 Goat-a-Mouse IgG diluted in normal goat serum for 1 h at room temperature. Personnel Communication. Kuldeep Patel, Loyola University.

Immunofluorescence

Immunofluorescence
Immunofluorescence microscopy of HeLa cells using anti-p53 a 1:100 dilution. Personnel Communication. Kuldeep Patel, Loyola University.

Western blot

Western blot
p53 Antibody - Western Blot. Western blotting using anti-p53. HeLa whole cell lysate (lane 1), cytosol fraction (lane 2) and nuclear extract (lane 3) (15 ug) were separated by 10% SDS-PAGE and transferred to nitrocellulose membrane. The membrane was blocked with 3% milk/TBST for 1 h at room temperature followed by incubation with Protein A purified Mab anti-p53 overnight at 4° C diluted 1:1500 in blocking solution. The membrane was washed 3X with TBST and then incubated with a 1:2000 dilution of HRP Goat-a-Mouse IgG diluted in blocking buffer for 1 h at room temperature. After final washes the proteins reactive on the membrane were detected using ECL. Other detection systems will yield similar results. Personnel Communication. Kuldeep Patel, Loyola University.

Western blot

Western blot
Western blotting using anti-p53. HeLa whole cell lysate (lane 1), cytosol fraction (lane 2) and nuclear extract (lane 3) (15 mg) incubated overnight with anti-p53 overnight at 4° C diluted 1:1,500. Personnel Communication. Kuldeep Patel, Loyola University.
 

Requested From: United States
Date Requested: 12/7/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number