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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TP53 / p53 Antibody (clone 5E2) LS-C172956

P53 antibody LS-C172956 is an unconjugated mouse monoclonal antibody to p53 (TP53) from human, mouse and monkey. Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C172956-100
100 µl (1 mg/ml)
$345
Description
P53 antibody LS-C172956 is an unconjugated mouse monoclonal antibody to p53 (TP53) from human, mouse and monkey. Validated for Flow, IF, IHC and WB.
Target
Human TP53 / p53
Host
Mouse
Reactivity
Human, Monkey, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
5E2
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Immunofluorescence (1:100)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
TP53 / p53 antibody was raised against full length human recombinant protein of human TP53 (NP_000537) produced in HEK293T cell.
Specificity
Human p53
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About TP53 / p53
P04637 NM_000546 NP_000537.3

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Images

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-TP53 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-TP53 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TP53 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TP53 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TP53 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TP53 mouse monoclonal antibody.

Immunofluorescence

Anti-TP53 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53.
Anti-TP53 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-TP53 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-TP53 monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-TP53 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-TP53 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TP53 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TP53 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TP53 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TP53 mouse monoclonal antibody.

Immunofluorescence

Anti-TP53 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53.
Anti-TP53 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-TP53 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-TP53 monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-TP53 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-TP53 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TP53 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TP53 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TP53 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TP53 mouse monoclonal antibody.

Immunofluorescence

Anti-TP53 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53.
Anti-TP53 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-TP53 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-TP53 monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-TP53 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-TP53 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TP53 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TP53 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TP53 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TP53 mouse monoclonal antibody.

Immunofluorescence

Anti-TP53 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53.
Anti-TP53 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-TP53 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-TP53 monoclonal antibody.

Requested From: United States
Date Requested: 4/20/2019
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