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Catalog Number Size Price
LS-C287179-10 10 µl $295 
LS-C287179-100 100 µl (1 mg/ml) $514 
TP53 / p53 Antibody - Detection of Human p53 by Immunohistochemistry. Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-p53 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
TP53 / p53 Antibody - Detection of human p53 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p53 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
TP53 / p53 Antibody - Detection of Human p53 by Western Blot. Samples: Whole cell lysate (100 ug) from 293T cells that were treated with an siRNA for p53 (+) or mock treated (-). Antibody: Rabbit anti-p53 antibody used at the indicated concentrations. Detection: Chemiluminescence with an exposure time of less than 1 minute.
TP53 / p53 Antibody - Detection of Human p53 by Immunohistochemistry. Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-p53 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
TP53 / p53 Antibody - Detection of human p53 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p53 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
TP53 / p53 Antibody - Detection of Human p53 by Western Blot. Samples: Whole cell lysate (100 ug) from 293T cells that were treated with an siRNA for p53 (+) or mock treated (-). Antibody: Rabbit anti-p53 antibody used at the indicated concentrations. Detection: Chemiluminescence with an exposure time of less than 1 minute.
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Polyclonal Rabbit anti‑Human TP53 / p53 Antibody (aa375‑C‑term, IHC, WB) LS‑C287179

Polyclonal Rabbit anti‑Human TP53 / p53 Antibody (aa375‑C‑term, IHC, WB) LS‑C287179

Antibody:
TP53 / p53 Rabbit anti-Human Polyclonal (aa375-C-term) Antibody
Application:
IHC, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295
LS-C287179-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TP53 / p53 Rabbit anti-Human Polyclonal (aa375-C-term) Antibody
Application:
IHC, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
P53 antibody LS-C287179 is an unconjugated rabbit polyclonal antibody to human p53 (TP53) (aa375-C-term). Validated for IHC, IP and WB.
Target
Human TP53 / p53
Synonyms
TP53 | BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 7157 corresponding to amino acids between 375 and the C-Terminus of UniprotID: P04637
Epitope
aa375-C-term
Specificity
Region between residues 375 and the C-terminus (residue 393) of human tumor protein p53 using the numbering given in SwissProt entry P04637 (GeneID 7157).
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (2 - 10 µg/mg lysate)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TP53 / p53
P04637 NM_000546 NP_000537.3

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024