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Antibodies > TP53 / p53 > LS-C353065
 
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Catalog Number Size Price
LS-C353065-100 100 µl (1 mg/ml) $299 
TP53 / p53 Antibody - Immunohistochemical analysis of p53 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
TP53 / p53 Antibody - Immunofluorescent analysis of p53 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
TP53 / p53 Antibody - Western blot analysis of p53 expression in A549 (A); HeLa (B); MDAMB231 (C); HepG2 (D) whole cell lysates.
TP53 / p53 Antibody - Immunohistochemical analysis of p53 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
TP53 / p53 Antibody - Immunofluorescent analysis of p53 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
TP53 / p53 Antibody - Western blot analysis of p53 expression in A549 (A); HeLa (B); MDAMB231 (C); HepG2 (D) whole cell lysates.
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Polyclonal Rabbit anti‑Human TP53 / p53 Antibody (C‑Terminus, IHC, IF, WB) LS‑C353065

Polyclonal Rabbit anti‑Human TP53 / p53 Antibody (C‑Terminus, IHC, IF, WB) LS‑C353065

Submit Review LSBio Guarantee
Antibody:
TP53 / p53 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C353065-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
TP53 / p53 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
P53 antibody LS-C353065 is an unconjugated rabbit polyclonal antibody to p53 (TP53) (C-Terminus) from human. It is reactive with human, rat and monkey. Validated for ICC, IF, IHC and WB.
Target
Human TP53 / p53
Synonyms
TP53 | BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
Host
Rabbit
Reactivity
Human, Monkey, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human p53.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of p53 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TP53 / p53
P04637 NM_000546 NP_000537.3

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IHC‑plus™ TP53 / p53 Antibody (clone BP53‑12) LS‑B431
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA, Chromatin Immunoprecipitation
TP53 / p53 Antibody - Colon carcinoma stained with anti-p53 antibody
TP53 / p53 Antibody LS‑C50534
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TP53 / p53 Antibody - Flow cytometry of TP53 / p53 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
TP53 / p53 Antibody (clone PAb240, Biotin) LS‑C134560
Species: Mouse, Human, Rat, Bovine, Hamster, Chicken
Applications: IHC, Western blot, Flow Cytometry
TP53 / p53 Antibody - Anti-TP53 / p53 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
TP53 / p53 Antibody (N‑Terminus) LS‑B4557
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Product
TP53 / p53 Antibody (aa20‑25, clone DO‑1) LS‑C11553
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Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
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Species: Human
Applications: IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
See all TP53 / p53 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
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