P53 antibody LS-C11556 is an unconjugated mouse monoclonal antibody to p53 (TP53) from human and bovine. Validated for Flow, IHC, IP and WB.
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (1:1000)
- IHC - Frozen
- Western blot (1:1000)
- Flow Cytometry (1:50 - 1:100)
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TP53 / p53 antibody was raised against recombinant human p53 ( AA 20-25 ).
Recognizes the human 53kD p53 tumor suppressor protein, also known as Cellular tumor antigen p53 or Antigen NY-CO-13, encoded by the TP53 gene. p53 is a 393 amino acid protein with an N-terminal transactivation domain, followed by a proline-rich region and a DNA binding domain in the central core region. The C-terminal region contains a tetramerization domain and a terminal regulatory domain. p53 is intimately involved in a number of signaling pathways controlling cell division, cycling and apoptosis and is thus a potent cancer suppressor. In normal cells the level of p53 expression is low but can be induced by DNA damage or other stress signals. Activation of p53 leads to growth arrest through its interaction with p21, GADD45 and, DNA repair and potentially apoptosis through interaction with,, and. p53 is critically regulated by Mdm2 which can trigger p53 degradation by a ubiquitin dependent systemrecognizes an epitope at the N-terminal end of p53 between amino acids 20-25, binding to both wild type and mutant forms. Clone DO-7 is not expected to recognize the multiple lacking the N-terminal region.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 whole blood in 100ul. Membrane permeabilization is required for this application. Immunohistochemistry: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. Histology Positive Control Tissue: Colon or breast carcinoma.
PBS, pH 7.4, 0.09% Sodium Azide
For research use only.