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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TP53 / p53 Antibody (aa20‑25, clone DO‑1) LS‑C11553
Note: This antibody replaces LS-C43831
P53 antibody LS-C11553 is an unconjugated mouse monoclonal antibody to p53 (TP53) from human, bovine, cat and other species. Validated for ELISA, IHC, IP and WB. Cited in 1 publication.
Antibody
Catalog
Size
Price
LS-C11553-100
100 µg (1 mg/ml)
$325

Popular TP53 / p53 Products

Anti-TP53 / p53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B431 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Colon carcinoma stained with anti-p53 antibody
Species: Human
Applications: IHC
Flow cytometry of TP53 / p53 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human, Rat, Bovine, Hamster, Chicken
Applications: IHC, Western blot, Flow Cytometry
Anti-TP53 / p53 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4557 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Bovine, Pig, Sheep
Applications: IHC, IHC - Paraffin, Western blot
Western blot analysis of UV-treated HEK 293 cell lysates using p53 Antibody (6F2D3), mAb, Mouse. The signal was developed with IRDye™ 800 Conjugated Goat Anti-Mouse IgG. Predicted Size: 53 KD Observed Size: 53 KD
Species: Human
Applications: Western blot, Flow Cytometry, ELISA

Product Description

P53 antibody LS-C11553 is an unconjugated mouse monoclonal antibody to p53 (TP53) from human, bovine, cat and other species. Validated for ELISA, IHC, IP and WB. Cited in 1 publication.
About TP53 / p53
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. P04637 NM_000546 NP_000537.3

TP53 Antibody, BCC7 Antibody, Cellular tumor antigen p53 Antibody, p53 Antibody, TRP53 Antibody, p53 tumor suppressor Antibody, Phosphoprotein p53 Antibody, Antigen NY-CO-13 Antibody, Tumor protein p53 Antibody, Tumor suppressor p53 Antibody, LFS1 Antibody


Specifications

Target
Human TP53 / p53
Host
Mouse
Reactivity
Human, Bovine, Cat, Horse (tested or 100% immunogen sequence identity)
Non-Reactivity
Mouse, Rat
Clonality
IgG2a Monoclonal [DO-1]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:25)
  • IHC - Frozen
  • Western blot (1:1000)
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
TP53 / p53 antibody was raised against recombinant human p53 ( AA 20-25 ).
Epitope
aa20-25
Specificity
Recognizes the human p53 tumor suppressor protein, also known as cellular tumor antigen p53 or NY-CO-13. Clone DO-1 binds to both wild type and mutant forms of the p53 protein found in various malignancies. p53 is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells and thus functions as a tumor suppressor that is involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancerrecognizes an epitope at the N-terminal end of p53 between amino acids 20-25, common to isoforms 1-3 of p53.
Usage
Immunohistology: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. We recommend the use of 0.01 M citrate buffer for this purpose.
Presentation
PBS, 0.09% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (1)

1
Effects of autophagy regulation of tumor-associated macrophages on radiosensitivity of colorectal cancer cells. Shao LN, Zhu BS, Xing CG, Yang XD, Young W, Cao JP. Molecular medicine reports. 2016 13:2661-70. (WB; Human) [Full Text Article] [PubMed:26821367]

Customer Reviews (0)


Requested From: United States
Date Requested: 8/18/2018

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