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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TP53 / p53 Antibody (aa20-25, clone DO-1)

Note: This antibody replaces LS-C43831
P53 antibody LS-C11553 is an unconjugated mouse monoclonal antibody to p53 (TP53) from human, bovine, cat and other species. Validated for ELISA, IHC, IP and WB. Cited in 1 publication.
Catalog
Size
Price
LS-C11553-0.1
0.1 mg
$325
Description
P53 antibody LS-C11553 is an unconjugated mouse monoclonal antibody to p53 (TP53) from human, bovine, cat and other species. Validated for ELISA, IHC, IP and WB. Cited in 1 publication.
Target
Human TP53 / p53
Host
Mouse
Reactivity
Human, Bovine, Cat, Horse (tested or 100% immunogen sequence identity)
Non-Reactivity
Mouse, Rat
Clonality
IgG2a Monoclonal [DO-1]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:25)
  • IHC - Frozen
  • Western blot (1:1000)
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
TP53 / p53 antibody was raised against recombinant human p53 ( AA 20-25 ).
Epitope
aa20-25
Specificity
Recognizes the human p53 tumor suppressor protein, also known as cellular tumor antigen p53 or NY-CO-13. Clone DO-1 binds to both wild type and mutant forms of the p53 protein found in various malignancies. p53 is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells and thus functions as a tumor suppressor that is involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancerrecognizes an epitope at the N-terminal end of p53 between amino acids 20-25, common to isoforms 1-3 of p53.
Usage
Immunohistology: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. We recommend the use of 0.01 M citrate buffer for this purpose.
Presentation
PBS, 0.09% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About TP53 / p53
P04637 NM_000546 NP_000537.3

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Publications (1)

1
Effects of autophagy regulation of tumor-associated macrophages on radiosensitivity of colorectal cancer cells. Shao LN, Zhu BS, Xing CG, Yang XD, Young W, Cao JP. Molecular medicine reports. 2016 13:2661-70. (WB; Human) [Full Text Article] [PubMed:26821367]

Customer Reviews (0)


Requested From: United States
Date Requested: 1/23/2019
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