P53 antibody LS-C11558 is an unconjugated mouse monoclonal antibody to human p53 (TP53). Validated for IHC, IP and WB.
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (1:1000)
- IHC - Frozen
- Western blot (1:1000)
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TP53 / p53 antibody was raised against recombinant human p53 ( AA 181-190 ).
Recognizes the human Cellular tumor antigen p53, also known as p53 tumor suppressor protein or NY-CO-13. p53 is a 393 amino acid ~53kD cytoplasmic/ nuclear protein up-regulated in response to DNA damage and is found in a wide variety of transformed cells. DO-11 binds to an epitope within the central region of p53 between amino acids defining a cryptic epitope exposed in unfolded/ denatured p53. Nine isoforms of human p53 are produced by alternative splicing and promoter usage, the epitope recognized by clone DO-11 is present in all isoforms. Recognizes mutant forms of p53 and has been used successfully for detection of p53 by western blotting.
Immunohistology: This product does require antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
PBS, pH 7.4, 0.09% Sodium Azide
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.