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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TP53 / p53 Antibody (aa181‑190, clone DO‑11) LS‑C11558
P53 antibody LS-C11558 is an unconjugated mouse monoclonal antibody to human p53 (TP53). Validated for IHC, IP and WB.
Catalog
Size
Price
LS-C11558-100
100 µg (1 mg/ml)
$395
Description
P53 antibody LS-C11558 is an unconjugated mouse monoclonal antibody to human p53 (TP53). Validated for IHC, IP and WB.
Target
Human TP53 / p53
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [DO-11]
Conjugations
Unconjugated
Purification
Purified IgG
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:1000)
  • IHC - Frozen
  • Western blot (1:1000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
TP53 / p53 antibody was raised against recombinant human p53 ( AA 181-190 ).
Epitope
aa181-190
Specificity
Recognizes the human Cellular tumor antigen p53, also known as p53 tumor suppressor protein or NY-CO-13. p53 is a 393 amino acid ~53kD cytoplasmic/ nuclear protein up-regulated in response to DNA damage and is found in a wide variety of transformed cells. DO-11 binds to an epitope within the central region of p53 between amino acids defining a cryptic epitope exposed in unfolded/ denatured p53. Nine isoforms of human p53 are produced by alternative splicing and promoter usage, the epitope recognized by clone DO-11 is present in all isoforms. Recognizes mutant forms of p53 and has been used successfully for detection of p53 by western blotting.
Usage
Immunohistology: This product does require antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About TP53 / p53
P04637 NM_000546 NP_000537.3

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Requested From: United States
Date Requested: 11/18/2018
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