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TOP2B / Topoisomerase II Beta Antibody (aa1548‑1597) LS‑C110235
Topoisomerase II Beta antibody LS-C110235 is an unconjugated rabbit polyclonal antibody to Topoisomerase II Beta (TOP2B) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C110235-50
50 µg
Unavailable

Popular TOP2B / Topoisomerase II Beta Products

Detection of Human Topo II Beta by Immunohistochemistry. Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:250.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence
Detection of Human Topo II Beta by Immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Detection of Human Topo II Beta by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemical analysis of Topoisomerase 2 beta staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Monkey, Dog, Pig
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA

Product Description

Topoisomerase II Beta antibody LS-C110235 is an unconjugated rabbit polyclonal antibody to Topoisomerase II Beta (TOP2B) from human, mouse, rat and other species. Validated for WB.
About TOP2B / Topoisomerase II Beta
TOP2B / Topoisomerase II Beta is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Q02880 NM_001068 NP_001059.2

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Specifications

Target
Human TOP2B / Topoisomerase II Beta
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Bat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, HRP, Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa1548-1597 of human TOP2B (Q02880, NP_001059). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Dog, Rabbit, Horse, Pig, Guinea pig (100%); Elephant, Bat (92%); Platypus (84%).
Blocking Peptide
LS-E13634 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110235. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1548-1597
Specificity
Human TOP2B
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

TOP2B antibody LS-C110235 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TOP2B antibody LS-C110235 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TOP2B antibody LS-C110235 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TOP2B antibody LS-C110235 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TOP2B antibody LS-C110235 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TOP2B antibody LS-C110235 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TOP2B antibody LS-C110235 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TOP2B antibody LS-C110235 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/18/2018

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