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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TOP2A / Topoisomerase II Alpha Antibody (clone 1C5) LS‑C179771
Topoisomerase II Alpha antibody LS-C179771 is an unconjugated mouse monoclonal antibody to human Topoisomerase II Alpha (TOP2A). Validated for Flow, ICC, IHC, IP and WB.
Catalog
Size
Price
LS-C179771-100
100 µg (1 mg/ml)
Unavailable

Popular TOP2A / Topoisomerase II Alpha Products

Antibody
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation, Flow Cytometry
Anti-TOP2A antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4433 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Anti-TOP2A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7670 dilution 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

Topoisomerase II Alpha antibody LS-C179771 is an unconjugated mouse monoclonal antibody to human Topoisomerase II Alpha (TOP2A). Validated for Flow, ICC, IHC, IP and WB.
About TOP2A / Topoisomerase II Alpha
TOP2A / Topoisomerase II Alpha is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. P11388 NM_001067 NP_001058.2

TOP2A Antibody, DNA topoisomerase II, 170 kD Antibody, TOP2 Antibody, DNA gyrase Antibody, DNA topoisomerase 2-alpha Antibody, TP2A Antibody


Specifications

Target
Human TOP2A / Topoisomerase II Alpha
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [1C5]
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1 - 5 µg/ml)
  • ICC (1 - 10 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • Immunoprecipitation
  • Flow Cytometry (0.1 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant Human DNA Topo II(C-terminal 182a. a.).
Specificity
Human TOP2A
Usage
IHC: Heat treatment is necessary for paraffin-embedded sections; IP 1-10 ug/200-300 ul of cell extract;
Presentation
PBS, pH 7.2, 50% glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunocytochemistry

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Immunohistochemistry

Immunocytochemistry

Western blot

Flow Cytometry



Requested From: 
Date Requested: 6/22/2018

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