Human TOP2A / Topoisomerase II Alpha

Rabbit

Human, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)

Sheep (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic peptide from the C-Terminus of human Topoisomerase II alpha (RKPSTSDDSDSNFEK, aa1466-1480 of UniProt P11388). Percent identity by BLAST analysis: Human, Ferret, Cat, Elephant, Rabbit, Horse, Pig, Dog (100%); Sheep (93%); Bovine, Bat (87%); Rat, Hamster, Opossum (80%); Mouse (73%).

aa1466-1480

Human TOP2A / Topoisomerase II Alpha

• Western blot (0.5 - 1 µg/ml)

The human topoisomerase II enzyme is encoded by a single-copy gene which is mapped to 17q21-q22. The TOP2A gene spans approximately 30 kb and contains 35 exons. Furthermore, DNA topoisomerases are enzymes that control and alter the topologic states of DNA in both prokaryotes and eukaryotes. Topoisomerase II from eukaryotic cells catalyzes the relaxation of supercoiled DNA molecules, catenation, decatenation, knotting, and unknotting of circular DNA. It appears likely that the reaction catalyzed by topoisomerase II involves the crossing-over of 2 DNA segments. There are about 100,000 molecules of topoisomerase II per HeLa cell nucleus, constituting about 0.1% of the nuclear extract. DNA topoisomerase II-alpha is associated with the pol II holoenzyme and is a required component of chromatin-dependent coactivation. Specific inhibitors of topoisomerase II blocked transcription on chromatin templates, but did not affect transcription on naked templates. Addition of purified topoisomerase II-alpha reconstituted chromatin-dependent activation activity in reactions with core pol II.

Lyophilized from PBS, 2.5% BSA, 0.025% sodium azide/thimerosal.

Reconstitute in sterile distilled water.

Prior to reconstitution, 4°C. Following reconstitution store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P11388 NCBI: NM_001067 NP_001058.2