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Anti-TOP1 / Topoisomerase I Antibody LS-C132023

Ordering

Wt. Vol. Conc. Price
- 2000 µl - $425
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular TOP1 / Topoisomerase I Antibodies

Anti-TOP1 / Topoisomerase I Antibody (clone 1A1) LS-C105043
Mouse Monoclonal [clone 1A1] (IgG1,k) to Human TOP1 / Topoisomerase I
Human
Western blot, ELISA
Unconjugated
Enzyme-Linked Immunosorbent Assay Image

100% Guaranteed 100% Guaranteed
Human Polyclonal to Human TOP1 / Topoisomerase I
Human
Immunofluorescence, Western blot, ELISA, Immunodiffusion, Radioimmunoassay
Unconjugated

Details

Human TOP1 / Topoisomerase I
Human
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Delipidated and defibrinated
Unmodified

Applications

  • Immunofluorescence
  • Western blot
  • ELISA
  • Immunodiffusion
  • Radioimmunoassay

Specificity and Use

TOP1 / Topoisomerase I antibody was raised against human Scl-70 Antigen from human plasma.
Recognizes human Scl-70 antigen. Does not recognize purified SSA, SSB, Sm, Sm/RNp and Jo-1 antigen.
Suitable for use in DNA conformational analysis and topology as well as DNA repair, drug resistance, cell proliferation and leukemia studies. The double-helical configuration that DNA strands naturally reside in makes them difficult to separate, and yet they must be separated if enzymes are to transcribe the sequences that encode proteins, or if chromosomes are to be replicated. In so-called circular DNA, in which double helical segment is bent around and joined in a circle, the two strands are topologically linked, or knotted. They cannot be separated by any process that does not involve the breaking of strands. Topoisomerases catalyze and guide the unknotting of DNA. Type I topoisomerases cut only one strand of DNA; type I topoisomerase of E. coli > E. coli (omega protein) relaxes negatively supercoiled DNA and does not act on positively supercoiled DNA. Type II topoisomerases cut both strands of DNA; type II topoisomerase of E. coli (DNA gyrase) increases the degree of negative supercoiling in DNA and requires ATP. It is inhibited by several antibiotics, including nalidixic acid and ovobiocin. Antibodies generated against the nuclear constituents are known as antinuclear antibodies (ANA). This includes autoantibodies directed against the extractable (soluble in physiological buffers) nuclear antigen or ENA. The most prominent of ANAs/ENAs are autoantibodies which binds to ds-DNA, ss-DNA, histones, ribonucleoproteins (RNP) and the SS-A, SS-B, Sm antigens, Jo-1, and Scl-70. Two antibodies, anti-dsDNA and anti-Sm, appear to occur only in SLE. Others occur in a variety of autoimmune and mixed connective tissue diseases. Antibody to Scl-70 antigen was originally called Scl-1. Anti-Scl-70 has been shown to react with a cellular antigen known as DNA topoisomerase I, which is responsible for the relaxation of supercoiled DNA. Anti-Scl-70 is found in ~75% patients with the diffuse progressive form of scleroderma. The presence of anti-Scl-70 seems to exclude the presence of anti-centromere antibody, which is a marker for a subset of patients with scleroderma known as CREST (calcinosis, sclerodactyly, and telangiectasia) syndrome. The frequency of ANA-positives in various rheumatic diseases has been reported for SLE, rheumatoid arthritis (RA), progressive systemic sclerosis (PSS), polymyositis (PM), dermatomyositis (DM), mixed connective tissue diseases, drug-induced SLE, and Sjogren's syndrome (SS). Most of these studies are based on tedious fluorescent ANA (FANA). Other techniques such as RIA, immunodiffusion, hemagglutination, electrophoresis and immunoblotting are also used to define antibody specificity. Recently, immunological assays (mostly ELISA) that determine the specificity of ANA have been used in studying patients with systemic rheumatic diseases. Applications. Suitable for use in ELISA and Immunodiffusion.

Packaging

Lyophilized from 0.01 M phosphate, 0.13 M sodium chloride, pH 7.2, 0.02% sodium azide
2,000 µl Sterile distilled water, sterile 40-50% glycerol.
Lyophilized powder may be stored at -20°C. Reconstituted product is stable for 12 months at -20°C.
For research use only.

About TOP1 / Topoisomerase I

P11387 NM_003286 NP_003277.1

TOP1 Antibody, DNA topoisomerase I Antibody, DNA topoisomerase 1 Antibody, TOPI Antibody, Topoisomerase (DNA) I Antibody, Topoisomerase I Antibody, Type I DNA topoisomerase Antibody

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand.

Requested From: United States
Date Requested: 12/9/2016

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