TOP1 / Topoisomerase I

topoisomerase (DNA) I

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

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Products

Antibodies (43)

ELISA Kits (9)

Proteins (5)

Type

Custom (1)

Over-Expression Lysate (2)

Primary (43)

Recombinant (3)

Sandwich (8)

Target

TOP1 / Topoisomerase I (57)

Reactivity

Human (46)

Mouse (16)

Rat (8)

Bat (3)

Bovine (3)

Chimpanzee (3)

Gibbon (3)

Guinea pig (3)

Hamster (3)

Horse (3)

Monkey (3)

Pig (3)

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Application

IHC (9)

IHC-P (4)

WB (37)

Flo (1)

ELISA (12)

ICC (5)

ID (1)

IF (10)

IP (7)

Peptide-ELISA (1)

Host

rabbit (36)

mouse (6)

human (1)

Product Group

IHCPlus (2)

Isotype

Ig (1)

IgG (20)

IgG1 (5)

IgG1,k (1)

Clonality

monoclonal mc (6)

polyclonal pc (37)

Clone

1291CT875.142.166 (1)

23B11 (1)

24.5 (1)

OTI3B9 (2)

Format

96-Well Strip Plate (9)

APC (1)

APC Conjugated (2)

Azide-free (1)

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Carrier-free (1)

Cy3 Conjugated (1)

Cy7 Conjugated (1)

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PE Conjugated (2)

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aa1-250 (8)

aa2-31 (7)

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Internal (1)

aa100-150 (1)

aa699-725 (1)

aa715-765 (1)

Publications

No (57)

Sample Type

Cell Lysates (3)

Plasma (4)

Serum (4)

Tissue Homogenates (5)

Tag

ABP-His, N-terminus (1)

GST, N-terminus (1)

His-T7 (1)

Myc-DDK (Flag) (2)

Species

Human (4)

Source

293T Cells (1)

E. coli (2)

HEK 293 Cells (1)

Wheat Germ Extract (1)

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TOP1 / Topoisomerase I Antibody LS-C132023

Antibody:

TOP1 / Topoisomerase I Human anti-Human Polyclonal Antibody

Reactivity:

Human

Applications:

ELISA, ID

Format:

Unconjugated

Price:

2 ml/$553

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Human TOP1 / Topoisomerase I (Sandwich ELISA) ELISA Kit - LS-F6459

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Human

Range:

0.156 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Cell Lysates, Tissue Homogenates

Price:

1 Plate/$834

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Human TOP1 / Topoisomerase I (Sandwich ELISA) ELISA Kit - LS-F12829

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Human

Range:

0.78 - 50 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

1 Plate/$783

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Mouse TOP1 / Topoisomerase I (Sandwich ELISA) ELISA Kit - LS-F12828

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Mouse

Range:

0.156 - 10 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

1 Plate/$783

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IHC-plus™ TOP1 / Topoisomerase I Antibody (aa715-765) LS-B11789

(4.0) Rating

Antibody:

TOP1 / Topoisomerase I Rabbit anti-Human Polyclonal (aa715-765) Antibody

Reactivity:

Mouse, Human

Applications:

IHC, IHC-P, IP, WB

Format:

Unconjugated

Price:

50 µl/$460

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TOP1 / Topoisomerase I Antibody (N-Terminus, clone 1291CT875.142.166) LS-C344125

Antibody:

TOP1 / Topoisomerase I Mouse anti-Human Monoclonal (N-Terminus) (1291CT875.142.166) Antibody

Reactivity:

Human

Applications:

Flo, WB

Format:

Unconjugated

Price:

400 µl/$393

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TOP1 / Topoisomerase I Antibody LS-C661168

Antibody:

TOP1 / Topoisomerase I Rabbit anti-Human Polyclonal Antibody

Reactivity:

Mouse, Human

Applications:

ELISA, IHC, IP, WB

Format:

Unconjugated

Price:

100 µg/$341

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TOP1 / Topoisomerase I Antibody (aa93-151, clone OTI3B9) LS-C795204

Antibody:

TOP1 / Topoisomerase I Mouse anti-Human Monoclonal (aa93-151) (OTI3B9) Antibody

Reactivity:

Human

Applications:

IHC, WB

Format:

Unconjugated

Price:

100 µl/$434

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TOP1 / Topoisomerase I Antibody (aa93-151, clone OTI3B9, Carrier-free) LS-C792283

Antibody:

TOP1 / Topoisomerase I Mouse anti-Human Monoclonal (aa93-151) (Carrier-free) (OTI3B9) Antibody

Reactivity:

Human

Applications:

IHC, WB

Format:

Unconjugated, Carrier-free

Price:

100 µg/$511

• Unconjugated
• Unconjugated, Carrier-free

• Unconjugated
• Unconjugated, Carrier-free

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TOP1 / Topoisomerase I Antibody LS-C662639

Antibody:

TOP1 / Topoisomerase I Rabbit anti-Human Polyclonal Antibody

Reactivity:

Human

Applications:

ELISA, WB

Format:

Unconjugated

Price:

10 µg/$318; 100 µg/$470

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IHC-plus™ TOP1 / Topoisomerase I Antibody (aa100-150) LS-B15072

(4.0) Rating

Antibody:

TOP1 / Topoisomerase I Rabbit anti-Human Polyclonal (aa100-150) Antibody

Reactivity:

Human

Applications:

IHC, IHC-P, IP, WB

Format:

Unconjugated

Price:

100 µl/$485

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TOP1 / Topoisomerase I Antibody (aa2-31, Biotin) LS-C271948

Antibody:

TOP1 / Topoisomerase I Rabbit anti-Human Polyclonal (aa2-31) (Biotin) Antibody

Reactivity:

Human

Applications:

ELISA, ICC, IF, WB

Format:

Biotin Conjugated

Price:

200 µl/$952

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TOP1 / Topoisomerase I Antibody (aa2-31, FITC) LS-C271949

Antibody:

TOP1 / Topoisomerase I Rabbit anti-Human Polyclonal (aa2-31) (FITC) Antibody

Reactivity:

Human

Applications:

ELISA, ICC, IF, WB

Format:

FITC Conjugated

Price:

200 µl/$952

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TOP1 / Topoisomerase I Antibody (aa2-31, APC) LS-C271946

Antibody:

TOP1 / Topoisomerase I Rabbit anti-Human Polyclonal (aa2-31) (APC) Antibody

Reactivity:

Human

Applications:

ELISA, ICC, IF, WB

Format:

APC Conjugated

Price:

200 µl/$952

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TOP1 / Topoisomerase I Antibody (aa2-31, Azide-free, HRP) LS-C271950

Antibody:

TOP1 / Topoisomerase I Rabbit anti-Human Polyclonal (aa2-31) (Azide-free) (HRP) Antibody

Reactivity:

Human

Applications:

ELISA, ICC, IF, WB

Format:

HRP Conjugated, Azide-free

Price:

200 µl/$952

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TOP1 / Topoisomerase I Antibody (aa2-31, PE) LS-C271952

Antibody:

TOP1 / Topoisomerase I Rabbit anti-Human Polyclonal (aa2-31) (PE) Antibody

Reactivity:

Human

Applications:

ELISA, ICC, IF, WB

Format:

PE Conjugated

Price:

200 µl/$952

• Biotin
• FITC
• APC
• HRP, Azide-free
• PE

• Biotin
• FITC
• APC
• More...
  • HRP, Azide-free
  • PE

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Human TOP1 / Topoisomerase I Protein (Recombinant His-T7) (aa1-250) - LS-G14851

Source:

E. coli

Tag:

His-T7

Molecular Weight:

34.0 kDa

Price:

10 µg/$283; 50 µg/$452; 100 µg/$722; 200 µg/$902; 1 mg/$2,016; 500 µg/$1,553; 5 mg/$3,151; 2 mg/$2,204

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TOP1 / Topoisomerase I Antibody LS-C827744

Antibody:

TOP1 / Topoisomerase I Rabbit anti-Human Polyclonal Antibody

Reactivity:

Mouse, Human

Applications:

ELISA, IHC, IP, WB

Format:

Unconjugated

Price:

100 µg/$341

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TOP1 / Topoisomerase I Antibody (N-Terminus, clone 24.5) LS-C354946

Antibody:

TOP1 / Topoisomerase I Mouse anti-Human Monoclonal (N-Terminus) (24.5) Antibody

Reactivity:

Human

Applications:

IF, IP, WB

Format:

Unconjugated

Price:

100 µg/$504

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TOP1 / Topoisomerase I Antibody LS-C805626

Antibody:

TOP1 / Topoisomerase I Rabbit anti-Human Polyclonal Antibody

Reactivity:

Mouse, Rat, Human

Applications:

IF, Peptide-ELISA, WB

Format:

Unconjugated

Price:

100 µl/$379; 200 µl/$421

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TOP1 / Topoisomerase I Antibody LS-C747620

Antibody:

TOP1 / Topoisomerase I Rabbit anti-Human Polyclonal Antibody

Reactivity:

Mouse, Human

Applications:

IHC, IP, WB

Format:

Unconjugated

Price:

20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503

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TOP1 / Topoisomerase I Antibody LS-C771321

Antibody:

TOP1 / Topoisomerase I Rabbit anti-Human Polyclonal Antibody

Reactivity:

Mouse, Human

Applications:

IHC, IP, WB

Format:

Unconjugated

Price:

60 µl/$331; 120 µl/$403; 200 µl/$529

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TOP1 / Topoisomerase I Antibody LS-C668482

Antibody:

TOP1 / Topoisomerase I Rabbit anti-Human Polyclonal Antibody

Reactivity:

Mouse, Human

Applications:

IHC, IHC-P, WB

Format:

Unconjugated

Price:

50 µl/$286; 100 µl/$321

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TOP1 / Topoisomerase I Antibody LS-C672732

Antibody:

TOP1 / Topoisomerase I Rabbit anti-Human Polyclonal Antibody

Reactivity:

Human

Applications:

ELISA, IF

Format:

Unconjugated

Price:

50 µg/$294; 100 µg/$360

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TOP1 / Topoisomerase I Antibody (HRP) LS-C672734

Antibody:

TOP1 / Topoisomerase I Rabbit anti-Human Polyclonal (HRP) Antibody

Reactivity:

Human

Format:

HRP Conjugated

Price:

50 µg/$294; 100 µg/$360

• Unconjugated
• HRP

• Unconjugated
• HRP

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