Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 23B11] (IgG1) to Human TOP1 / Topoisomerase I
Human TOP1 / Topoisomerase I
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [23B11]
Western blot (0.5 µg/ml)
Specificity and Use
TOP1 / Topoisomerase I antibody was raised against synthetic peptide corresponding to aa 699-725 of human topoisomerase 1.
Recognizes human topoisomerase I.
Suitable for use in Western Blot. Western Blot: 0.5 ug/ml using HRP/ECL for detection. Recommended blocking buffer: Casein/Tween 20-based blocking and blot incubation buffer. Positive control: Cell lysate from untreated HepG2 cells.
TOP1 Antibody, DNA topoisomerase I Antibody, DNA topoisomerase 1 Antibody, TOPI Antibody, Topoisomerase (DNA) I Antibody, Topoisomerase I Antibody, Type I DNA topoisomerase Antibody
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand.