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Anti-TNNT1 / TNT Antibody (C-Terminus) LS-C101944

Ordering

Wt. Vol. Conc. Price
50 µg - - Unavailable

Most Popular TNNT1 / TNT Antibodies

Anti-TNNT1 / TNT Antibody (aa205-218) IHC-plus™ LS-B10398
Goat Polyclonal to Human TNNT1 / TNT
Human
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-TNNT1 / TNT Antibody (aa1-278) LS-C296815
Rabbit Polyclonal (IgG) to Human TNNT1 / TNT
Human
Western blot, ELISA
Unconjugated
Western blot Image
Anti-TNNT1 / TNT Antibody (aa1-261) LS-C296816
Rabbit Polyclonal (IgG) to Mouse TNNT1 / TNT
Mouse
Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human TNNT1 / TNT
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human TNNT1 / TNT
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig (tested or 100% immunogen sequence identity)
Xenopus (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

TNNT1 / TNT antibody was raised against synthetic peptide from C-Terminus of human TNNT1 (P13805, NP_003274). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Horse, Pig, Guinea pig (100%); Xenopus (92%); Platypus (85%).
LS-E15501 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101944. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
C-Terminus
Human TNNT1
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About TNNT1 / TNT

AJ011713

TNNT1 Antibody, STNT Antibody, TNT Antibody, TNTS Antibody, Troponin-T1, skeletal, slow Antibody, ANM Antibody, Troponin T1, skeletal, slow Antibody, NEM5 Antibody

TNNT1 / TNT is a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit.

Western blot

Western blot
TNNT1 antibody LS-C101944 Western blot of Fetal Muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: 
Date Requested: 3/26/2017

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