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| Catalog Number | Size | Price |
|---|---|---|
| LS-B10398-50 | 50 µg (0.5 mg/ml) | $485 |
1 of 2
2 of 2
IHC‑plus™ Polyclonal Goat anti‑Human TNNT1 / TNT Antibody (aa205‑218, IHC, WB) LS‑B10398
IHC‑plus™ Polyclonal Goat anti‑Human TNNT1 / TNT Antibody (aa205‑218, IHC, WB) LS‑B10398
Note: This antibody replaces LS-C186617
Antibody:
TNNT1 / TNT Goat anti-Human Polyclonal (aa205-218) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
TNNT1 / TNT Goat anti-Human Polyclonal (aa205-218) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
TNT antibody LS-B10398 is an unconjugated goat polyclonal antibody to human TNT (TNNT1) (aa205-218). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TNNT1 / TNT
Synonyms
TNNT1 | STNT | TNT | TNTS | Troponin-T1, skeletal, slow | ANM | Troponin T1, skeletal, slow | NEM5
Host
Goat
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-EKAQELSDWIHQLE, from the internal region of the protein sequence according to NP_003274.3NP_001119604.1NP_001119605.1.
Epitope
aa205-218
Specificity
Human TNNT1 / TNT. This antibody is expected to recognize all reported isoforms (NP_003274.3; NP_001119604.1; NP_001119605.1).
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.03 - 0.1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments gave an approx 50kD band in Human Skeletal Muscle lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.9kD according to NP_003274.3. The 50kD band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TNNT1 / TNT
Validation
Anti-TNNT1 / TNT antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TNNT1 / TNT antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ TNNT1 / TNT Antibody (aa205-218) for IHC, WB/Western LS-B10398 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human, Mouse, Rat
Applications:
Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Reactivity:
Porcine
Range:
31.25-2000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/17/2024
Date Requested: 4/17/2024
