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TNNI3K / CARK Antibody (aa755‑804) LS‑C102284 Best Seller
CARK antibody LS-C102284 is an unconjugated rabbit polyclonal antibody to CARK (TNNI3K) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C102284-50
50 µg
Unavailable

Popular TNNI3K / CARK Products

Immunohistochemical analysis of CARK staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, Western blot
Western blot of TNNI3K antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA
Immunohistochemistry of paraffin-embedded human heart tissue at dilution 1:100
Species: Human
Applications: IHC, Western blot, ELISA
Immunohistochemistry of paraffin-embedded human heart tissue at dilution 1:100
Species: Human
Applications: IHC, ELISA

Product Description

CARK antibody LS-C102284 is an unconjugated rabbit polyclonal antibody to CARK (TNNI3K) from human, mouse, rat and other species. Validated for WB.
About TNNI3K / CARK
May play a role in cardiac physiology. Q59H18 NM_015978 NP_057062.1

TNNI3K Antibody, Cardiac ankyrin repeat kinase Antibody, CARK Antibody, TNNI3-interacting kinase Antibody, TNNI3 interacting kinase Antibody


Specifications

Target
Human TNNI3K / CARK
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Horse, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Chicken, Zebrafish (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
TNNI3K / CARK antibody was raised against synthetic peptide located between aa755-804 of human TNNI3K (Q59H18-2, NP_057062). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Rabbit, Horse, Opossum, Platypus (100%); Chicken, Zebrafish (92%); Xenopus (84%).
Blocking Peptide
LS-E17387 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C102284. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa755-804
Specificity
Human TNNI3K
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

TNNI3K antibody LS-C102284 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TNNI3K antibody LS-C102284 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TNNI3K antibody LS-C102284 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TNNI3K antibody LS-C102284 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TNNI3K antibody LS-C102284 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TNNI3K antibody LS-C102284 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

TNNI3K antibody LS-C102284 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TNNI3K antibody LS-C102284 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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