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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TNNI3 / Cardiac Troponin I Antibody (clone 4E5) LS‑C114351
Cardiac Troponin I antibody LS-C114351 is an unconjugated mouse monoclonal antibody to Cardiac Troponin I (TNNI3) from human, dog and monkey. Validated for IF and WB.
Catalog
Size
Price
LS-C114351-100
100 µl (1 mg/ml)
Unavailable

Popular TNNI3 / Cardiac Troponin I Products

Anti-TNNI3 / CTnI antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2268 concentration 5 ug/ml.
Species: Bovine, Human, Mouse, Rabbit, Chicken, Frog
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, Immunoprecipitation, ELISA
Anti-TNNI3 / CTnI antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3659 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

Cardiac Troponin I antibody LS-C114351 is an unconjugated mouse monoclonal antibody to Cardiac Troponin I (TNNI3) from human, dog and monkey. Validated for IF and WB.
About TNNI3 / Cardiac Troponin I
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. P19429 NM_000363 NP_000354.4

TNNI3 Antibody, CTnI Antibody, CMD1FF Antibody, CMD2A Antibody, RCM1 Antibody, Troponin I, cardiac Antibody, Troponin I, cardiac muscle Antibody, Troponin I type 3 (cardiac) Antibody, Cardiac troponin I Antibody, CMH7 Antibody


Specifications

Target
Human TNNI3 / Cardiac Troponin I
Host
Mouse
Reactivity
Human, Monkey, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [4E5]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:2500 - 1:5000)
Immunogen
Full length human recombinant protein of human TNNI3 (NP_000354) produced in E. coli.
Specificity
Human Cardiac Troponin I / TNNI3
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Western blot of 35 ug of cell extracts from human (HeLa) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human (HeLa) cells using anti-TNNI3 antibody.

Immunofluorescence

Western blot of 35 ug of cell extracts from human colon adenocarcinoma (HT29) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human colon adenocarcinoma (HT29) cells using anti-TNNI3 antibody.

Immunofluorescence

Western blot of 35 ug of cell extracts from human Lung adenocarcinoma (A549) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human Lung adenocarcinoma (A549) cells using anti-TNNI3 antibody.

Western blot

Western blot of 35 ug of cell extracts from Rat (PC12) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from Rat (PC12) cells using anti-TNNI3 antibody.

Western blot

Western blot of 35 ug of cell extracts from human breast adenocarcinoma (MCF-7) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human breast adenocarcinoma (MCF-7) cells using anti-TNNI3 antibody.

Western blot

Western blot of 35 ug of cell extracts from human brain tissue lysates using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human brain tissue lysates using anti-TNNI3 antibody.

Western blot

Western blot of 35 ug of cell extracts from canine Kidney (MDCK) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from canine Kidney (MDCK) cells using anti-TNNI3 antibody.

Western blot

Western blot of 35 ug of cell extracts from human Liver carcinoma (HepG2) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human Liver carcinoma (HepG2) cells using anti-TNNI3 antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNNI3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNNI3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNNI3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNNI3.

Western blot

Western blot of 35 ug of cell extracts from Green monkey Kidney (COS7) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from Green monkey Kidney (COS7) cells using anti-TNNI3 antibody.

Immunofluorescence

Western blot of 35 ug of cell extracts from human (HeLa) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human (HeLa) cells using anti-TNNI3 antibody.

Immunofluorescence

Western blot of 35 ug of cell extracts from human colon adenocarcinoma (HT29) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human colon adenocarcinoma (HT29) cells using anti-TNNI3 antibody.

Immunofluorescence

Western blot of 35 ug of cell extracts from human Lung adenocarcinoma (A549) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human Lung adenocarcinoma (A549) cells using anti-TNNI3 antibody.

Western blot

Western blot of 35 ug of cell extracts from Rat (PC12) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from Rat (PC12) cells using anti-TNNI3 antibody.

Western blot

Western blot of 35 ug of cell extracts from human breast adenocarcinoma (MCF-7) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human breast adenocarcinoma (MCF-7) cells using anti-TNNI3 antibody.

Western blot

Western blot of 35 ug of cell extracts from human brain tissue lysates using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human brain tissue lysates using anti-TNNI3 antibody.

Western blot

Western blot of 35 ug of cell extracts from canine Kidney (MDCK) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from canine Kidney (MDCK) cells using anti-TNNI3 antibody.

Western blot

Western blot of 35 ug of cell extracts from human Liver carcinoma (HepG2) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human Liver carcinoma (HepG2) cells using anti-TNNI3 antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNNI3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNNI3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNNI3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNNI3.

Western blot

Western blot of 35 ug of cell extracts from Green monkey Kidney (COS7) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from Green monkey Kidney (COS7) cells using anti-TNNI3 antibody.

Immunofluorescence

Western blot of 35 ug of cell extracts from human (HeLa) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human (HeLa) cells using anti-TNNI3 antibody.

Immunofluorescence

Western blot of 35 ug of cell extracts from human colon adenocarcinoma (HT29) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human colon adenocarcinoma (HT29) cells using anti-TNNI3 antibody.

Immunofluorescence

Western blot of 35 ug of cell extracts from human Lung adenocarcinoma (A549) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human Lung adenocarcinoma (A549) cells using anti-TNNI3 antibody.

Western blot

Western blot of 35 ug of cell extracts from Rat (PC12) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from Rat (PC12) cells using anti-TNNI3 antibody.

Western blot

Western blot of 35 ug of cell extracts from human breast adenocarcinoma (MCF-7) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human breast adenocarcinoma (MCF-7) cells using anti-TNNI3 antibody.

Western blot

Western blot of 35 ug of cell extracts from human brain tissue lysates using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human brain tissue lysates using anti-TNNI3 antibody.

Western blot

Western blot of 35 ug of cell extracts from canine Kidney (MDCK) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from canine Kidney (MDCK) cells using anti-TNNI3 antibody.

Western blot

Western blot of 35 ug of cell extracts from human Liver carcinoma (HepG2) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human Liver carcinoma (HepG2) cells using anti-TNNI3 antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNNI3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNNI3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNNI3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNNI3.

Western blot

Western blot of 35 ug of cell extracts from Green monkey Kidney (COS7) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from Green monkey Kidney (COS7) cells using anti-TNNI3 antibody.

Immunofluorescence

Western blot of 35 ug of cell extracts from human (HeLa) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human (HeLa) cells using anti-TNNI3 antibody.

Immunofluorescence

Western blot of 35 ug of cell extracts from human colon adenocarcinoma (HT29) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human colon adenocarcinoma (HT29) cells using anti-TNNI3 antibody.

Immunofluorescence

Western blot of 35 ug of cell extracts from human Lung adenocarcinoma (A549) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human Lung adenocarcinoma (A549) cells using anti-TNNI3 antibody.

Western blot

Western blot of 35 ug of cell extracts from Rat (PC12) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from Rat (PC12) cells using anti-TNNI3 antibody.

Western blot

Western blot of 35 ug of cell extracts from human breast adenocarcinoma (MCF-7) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human breast adenocarcinoma (MCF-7) cells using anti-TNNI3 antibody.

Western blot

Western blot of 35 ug of cell extracts from human brain tissue lysates using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human brain tissue lysates using anti-TNNI3 antibody.

Western blot

Western blot of 35 ug of cell extracts from canine Kidney (MDCK) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from canine Kidney (MDCK) cells using anti-TNNI3 antibody.

Western blot

Western blot of 35 ug of cell extracts from human Liver carcinoma (HepG2) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from human Liver carcinoma (HepG2) cells using anti-TNNI3 antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNNI3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNNI3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNNI3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNNI3.

Western blot

Western blot of 35 ug of cell extracts from Green monkey Kidney (COS7) cells using anti-TNNI3 antibody.
Western blot of 35 ug of cell extracts from Green monkey Kidney (COS7) cells using anti-TNNI3 antibody.


Requested From: 
Date Requested: 6/18/2018

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