Target
Human TNNI3 / Cardiac Troponin I
Synonyms
TNNI3 | CTnI | CMD1FF | CMD2A | RCM1 | Troponin I, cardiac | Troponin I, cardiac muscle | Troponin I type 3 (cardiac) | Cardiac troponin I | CMH7
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
Phosphorylated form of human cardiac Troponin I.
Specificity
Recognizes the phosphorylated form of human cardiac Troponin I (cTnI). Troponin I is a 24kD subunit of Troponin, a protein found on muscle tissue responsible for muscle contraction. Recognizes free cTnI and cTnI in complex with other Troponin components. It does not cross-react with skeletal Troponin I.
Usage
Western Blot: For the detection of phosphoproteins, phosphatase inhibitors such as 10 mM Sodium Fluoride and 1 mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Presentation
PBS, 0.09% Sodium Azide
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNNI3 / Cardiac Troponin I