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Catalog Number Size Price
LS-C186615-100 100 µg (0.5 mg/ml) $503 
TNNI3 / Cardiac Troponin I Antibody - TNNI3 / Cardiac Troponin I antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
TNNI3 / Cardiac Troponin I Antibody - TNNI3 / Cardiac Troponin I antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
TNNI3 / Cardiac Troponin I Antibody - TNNI3 / Cardiac Troponin I antibody (0.1µg/ml) staining of Human (A), (0.03µg/ml) Mouse (B), (0.1µg/ml) Rat (C) and Pig (D) Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
TNNI3 / Cardiac Troponin I Antibody - TNNI3 antibody (0.2 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TNNI3 / Cardiac Troponin I Antibody - TNNI3 / Cardiac Troponin I antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
TNNI3 / Cardiac Troponin I Antibody - TNNI3 / Cardiac Troponin I antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
TNNI3 / Cardiac Troponin I Antibody - TNNI3 / Cardiac Troponin I antibody (0.1µg/ml) staining of Human (A), (0.03µg/ml) Mouse (B), (0.1µg/ml) Rat (C) and Pig (D) Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
TNNI3 / Cardiac Troponin I Antibody - TNNI3 antibody (0.2 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Polyclonal Goat anti‑Human TNNI3 / Cardiac Troponin I Antibody (aa117‑127, IF, WB) LS‑C186615

Polyclonal Goat anti‑Human TNNI3 / Cardiac Troponin I Antibody (aa117‑127, IF, WB) LS‑C186615

Antibody:
TNNI3 / Cardiac Troponin I Goat anti-Human Polyclonal (aa117-127) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C186615-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TNNI3 / Cardiac Troponin I Goat anti-Human Polyclonal (aa117-127) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
Cardiac Troponin I antibody LS-C186615 is an unconjugated goat polyclonal antibody to Cardiac Troponin I (TNNI3) (aa117-127) from human. It is reactive with human, mouse, rat and other species. Validated for IF, Peptide-ELISA and WB.
Target
Human TNNI3 / Cardiac Troponin I
Synonyms
TNNI3 | CTnI | CMD1FF | CMD2A | RCM1 | Troponin I, cardiac | Troponin I, cardiac muscle | Troponin I type 3 (cardiac) | Cardiac troponin I | CMH7
Host
Goat
Reactivity
Human, Mouse, Rat, Bovine, Dog, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-KVTKNITEIAD, from the internal region of the protein sequence according to NP_000354.4.
Epitope
aa117-127
Specificity
Human TNNI3 / CTnI
Applications
  • Immunofluorescence (10 µg/ml)
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 27+28kD bands observed in Human Heart lysates (calculated MW of 24.0kD according to NP_000354.4). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.2-0.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNNI3 / Cardiac Troponin I
P19429 NM_000363 NP_000354.4

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Requested From: United States
Date Requested: 4/19/2024