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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TNNI2 Antibody (clone 2F12A11) LS‑C169425
TNNI2 antibody LS-C169425 is an unconjugated mouse monoclonal antibody to human TNNI2. Validated for ELISA, Flow and IHC.
Catalog
Size
Price
LS-C169425-100
100 µl
Unavailable

Popular TNNI2 Products

Anti-TNNI2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4963 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-TNNI2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7284 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA
TNNI2 antibody (0.03 ug/ml) staining of Human (A), Mouse(B) and Rat (C) Skeletal Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Species: Human, Mouse, Rat, Dog, Guinea pig, Hamster, Rabbit
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Mouse, Rat
Applications: IHC, Western blot
Antibody
Species: Mouse, Rat
Applications: IHC, Western blot

Product Description

TNNI2 antibody LS-C169425 is an unconjugated mouse monoclonal antibody to human TNNI2. Validated for ELISA, Flow and IHC.
About TNNI2
TNNI2 is a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. Mouse studies show that this component is also present in vascular smooth muscle and may play a role in regulation of smooth muscle function. P48788 NM_003282 NP_003273.1

TNNI2 Antibody, AMCD2B Antibody, DA2B Antibody, FSSV Antibody, FsTnI Antibody, Troponin I fast twitch 2 Antibody, Troponin I, skeletal, fast Antibody


Specifications

Target
Human TNNI2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [2F12A11]
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200 - 1:1000)
  • Flow Cytometry (1:200 - 1:400)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
TNNI2 antibody was raised against purified recombinant fragment of human TNNI2 expressed in E. Coli.\
Specificity
Human
Presentation
Ascitic fluid containing 0.03% sodium azide
Storage
Short term: 4°C; Long term: -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded rabbit cardiac muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded rabbit cardiac muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded cardiac muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded cardiac muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded striated muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded striated muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.

Western blot

Western blot using TNNI2 monoclonal antibody against human TNNI2 (AA: 1-182) recombinant protein. (Expected MW is 46.8 kDa)
Western blot using TNNI2 monoclonal antibody against human TNNI2 (AA: 1-182) recombinant protein. (Expected MW is 46.8 kDa)

Western blot

Western blot using TNNI2 monoclonal antibody against HEK293 (1) and TNNI2(AA: 1-182)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using TNNI2 monoclonal antibody against HEK293 (1) and TNNI2(AA: 1-182)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded rabbit cardiac muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded rabbit cardiac muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded cardiac muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded cardiac muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded striated muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded striated muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.

Western blot

Western blot using TNNI2 monoclonal antibody against human TNNI2 (AA: 1-182) recombinant protein. (Expected MW is 46.8 kDa)
Western blot using TNNI2 monoclonal antibody against human TNNI2 (AA: 1-182) recombinant protein. (Expected MW is 46.8 kDa)

Western blot

Western blot using TNNI2 monoclonal antibody against HEK293 (1) and TNNI2(AA: 1-182)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using TNNI2 monoclonal antibody against HEK293 (1) and TNNI2(AA: 1-182)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded rabbit cardiac muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded rabbit cardiac muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded cardiac muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded cardiac muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded striated muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded striated muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.

Western blot

Western blot using TNNI2 monoclonal antibody against human TNNI2 (AA: 1-182) recombinant protein. (Expected MW is 46.8 kDa)
Western blot using TNNI2 monoclonal antibody against human TNNI2 (AA: 1-182) recombinant protein. (Expected MW is 46.8 kDa)

Western blot

Western blot using TNNI2 monoclonal antibody against HEK293 (1) and TNNI2(AA: 1-182)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using TNNI2 monoclonal antibody against HEK293 (1) and TNNI2(AA: 1-182)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded rabbit cardiac muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded rabbit cardiac muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded cardiac muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded cardiac muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded striated muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded striated muscle tissues using TNNI2 mouse monoclonal antibody with DAB staining.

Western blot

Western blot using TNNI2 monoclonal antibody against human TNNI2 (AA: 1-182) recombinant protein. (Expected MW is 46.8 kDa)
Western blot using TNNI2 monoclonal antibody against human TNNI2 (AA: 1-182) recombinant protein. (Expected MW is 46.8 kDa)

Western blot

Western blot using TNNI2 monoclonal antibody against HEK293 (1) and TNNI2(AA: 1-182)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using TNNI2 monoclonal antibody against HEK293 (1) and TNNI2(AA: 1-182)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);


Requested From: 
Date Requested: 6/22/2018

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